Purified ribonuclease inhibitor tested to be free of contaminating activity for the degradation of nucleic acids.

• Unit Definition: One unit will inhibit 50% of the activity of 5 ng of RNase A
• RNase Block does not inhibit RNase H, RNase T1, T7, T3, or SP6 RNA polymerases, Taq DNA polymerase, AMV or MMLV reverse transcriptases

RNase block ribonuclease inhibitor is a 50 kDa protein that specifically inhibits common eukaryotic RNases including RNase A, RNase B, and RNase C. It is a recombinant ribonuclease inhibitor derived by a molecular cloning technology that inactivates RNases by binding noncovalently to the RNase molecule. Only 25 to 40 U is required for a standard transcription reaction.

Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts with 40 U of RNase Block Ribonuclease Inhibitor for 1 hour at 37 °C and by checking for the absence of degradation.

Applications: RNA transcription, in vitro RNA translation, RNA capping, and Any application where eukaryotic RNase contamination is a potential problem.