VWR®, Taq DNA polymerase

Certifikater SDS

Leverandør: VWR Chemicals

VWR®, <i>Taq</i> DNA polymerase

Leverandør: VWR Chemicals

Certifikater

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Specifications for Product Family Product Family Options

Om denne vare

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

Ideal choice for routine applications
High performance, thermostable DNA polymerase
Optimal for TA cloning

Taq DNA polymerase concentration: 5 Units/μl

10X Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8,3; KCl, 15 mM MgCl₂, 1% Triton™ X-100
10X Mg-Free Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 1% Tween® 20
10X Mg-Free Extra Buffer: Tris-HCl pH 8,3; KCl, 1% Triton™ X-100

EU = Units

Leveringsinformation: VWR® Taq DNA Polymerase is usually supplied with either or both Key Buffer and Extra Buffer. Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimising the need for optimisation of the Mg²⁺ concentration, or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimisation of primer annealing temperatures and Mg²⁺ concentrations may be required.

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Product Details & Documents

Ideal choice for routine applications
High performance, thermostable DNA polymerase
Optimal for TA cloning

Taq DNA polymerase concentration: 5 Units/μl

EU = Units

Documents

Certifikater

Søg efter partinummer

Produktlitteratur

Avantor PCR Solutions
VWR® for PCR

Fast PCR protocols for VWR® Taq DNA Polymerase

SDS

Datablad

VWR® PCR Enzymes

Specifications for Product Family

Specifikationer

Catalog No:
733-1300
733-1301
733-1302
733-1819
733-1820
733-1303
733-1312
733-1313
733-2009
733-1305
733-1307
733-1823

Beskrivelse:
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Taq DNA-polymerase, 10X MgCl₂-fri Extra Buffer, 25 mM MgCl₂
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂

Format:
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer
Enzyme with/without buffer

Funktioner:
Mg-free
Mg-free
Mg-free
Detergent free
Detergent free
Detergent free

Varmstart:
No
No
No
No
No
No
No
No
No
No
No
No

Koncentration:
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl
5 U/µl

Enheder:
250
500
1000
2500
5000
10000
1000
2500
10000
1000
1000
10000

Størrelse:
1 x 0.05 ml + 3 x 1.5 ml
1 x 0.1 ml + 3 x 1.5 ml
2 x 0.1 ml + 6 x 1.5 ml
5 x 0.1 ml + 15 x 1.5 ml
10 x 0.1 ml + 9 x 5 ml
3 x 0.667 ml + 18 x 5 ml
2 x 0.1 ml + 4 x 1.5 ml
5 x 0.1 ml + 10 x 1.5 ml
3 x 0.667 ml + 12 x 5 ml
2 x 0.1 ml + 4 x 1.5 ml
2 x 0.1 ml + 4 x 1.5 ml
3 x 0.667 ml + 18 x 5 ml

Buffer sammensætning:
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
10x Key Buffer Mg-free and 25 mM MgCl2
10x Key Buffer Mg-free and 25 mM MgCl2
10x Key Buffer Mg-free and 25 mM MgCl2
10x Extra Buffer and 25 mM MgCl2
Detergent free
10x Key Buffer and 25 mM MgCl2
Detergent free
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Detergent free

Høj troskab:
No
No
No
No
No
No
No
No
No
No
No
No

Amplikon størrelse:
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb
≤ 5kb

Processivity:
Standard
Standard
Standard
Standard
Standard
Standard
Standard
Standard
Standard
Standard
Standard
Standard

Exonuklease aktivitet:
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’
5’→3’

Product overhang:
3'A
3'A
3'A
3'A
3'A
3'A
3'A
3'A
3'A
3'A
3'A
3'A

Opbevaring betingelser:
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)
– 20 °C (long term), + 4 °C (6 months)

Ansøgning:
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification
Standard amplification

Specifikationer

Product Family Options

Produktinformation

Pakn
Tilgængelighed
Price
733-1300
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Certifikater SDS
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
250 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 250 EE
1 * 250 EE - kr702.00
kr702.00
1 * 250 EE
kr702.00
Specifikationer:
More
Specifications
Cat. No.
733-1300
Beskrivelse
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Varmstart
No
Koncentration
5 U/µl
Enheder
250
Størrelse
1 x 0.05 ml + 3 x 1.5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1301
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Certifikater SDS
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
500 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 500 EE
1 * 500 EE - kr1,290.00
kr1,290.00
1 * 500 EE
kr1,290.00
Specifikationer:
More
Specifications
Cat. No.
733-1301
Beskrivelse
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Varmstart
No
Koncentration
5 U/µl
Enheder
500
Størrelse
1 x 0.1 ml + 3 x 1.5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1302
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Certifikater SDS
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
1.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 1.000 EE
1 * 1.000 EE - kr2,610.00
kr2,610.00
1 * 1.000 EE
kr2,610.00
Specifikationer:
More
Specifications
Cat. No.
733-1302
Beskrivelse
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Varmstart
No
Koncentration
5 U/µl
Enheder
1000
Størrelse
2 x 0.1 ml + 6 x 1.5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1819
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Certifikater
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
2.500 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 2.500 EE
1 * 2.500 EE - kr4,140.00
kr4,140.00
1 * 2.500 EE
kr4,140.00
Specifikationer:
More
Specifications
Cat. No.
733-1819
Beskrivelse
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Varmstart
No
Koncentration
5 U/µl
Enheder
2500
Størrelse
5 x 0.1 ml + 15 x 1.5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1820
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Certifikater SDS
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
5.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 5.000 EE
1 * 5.000 EE - kr7,500.00
kr7,500.00
1 * 5.000 EE
kr7,500.00
Specifikationer:
More
Specifications
Cat. No.
733-1820
Beskrivelse
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Varmstart
No
Koncentration
5 U/µl
Enheder
5000
Størrelse
10 x 0.1 ml + 9 x 5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1303
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
SDS
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
10.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 10.000 EE
1 * 10.000 EE - kr18,100.00
kr18,100.00
1 * 10.000 EE
kr18,100.00
Specifikationer:
More
Specifications
Cat. No.
733-1303
Beskrivelse
Taq DNA-polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Varmstart
No
Koncentration
5 U/µl
Enheder
10000
Størrelse
3 x 0.667 ml + 18 x 5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1312
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Certifikater SDS
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
1.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 1.000 EE
1 * 1.000 EE - kr2,630.00
kr2,630.00
1 * 1.000 EE
kr2,630.00
Specifikationer:
More
Specifications
Cat. No.
733-1312
Beskrivelse
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Mg-free
Varmstart
No
Koncentration
5 U/µl
Enheder
1000
Størrelse
2 x 0.1 ml + 4 x 1.5 ml
Buffer sammensætning
10x Key Buffer Mg-free and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1313
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
SDS
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
2.500 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 2.500 EE
1 * 2.500 EE - kr5,200.00
kr5,200.00
1 * 2.500 EE
kr5,200.00
Specifikationer:
More
Specifications
Cat. No.
733-1313
Beskrivelse
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Mg-free
Varmstart
No
Koncentration
5 U/µl
Enheder
2500
Størrelse
5 x 0.1 ml + 10 x 1.5 ml
Buffer sammensætning
10x Key Buffer Mg-free and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-2009
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Certifikater SDS
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
10.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 10.000 EE
1 * 10.000 EE - kr18,280.00
kr18,280.00
1 * 10.000 EE
kr18,280.00
Specifikationer:
More
Specifications
Cat. No.
733-2009
Beskrivelse
Taq DNA-polymerase, 10X MgCl₂-fri Key Buffer, 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Mg-free
Varmstart
No
Koncentration
5 U/µl
Enheder
10000
Størrelse
3 x 0.667 ml + 12 x 5 ml
Buffer sammensætning
10x Key Buffer Mg-free and 25 mM MgCl2
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1305
Taq DNA-polymerase, 10X MgCl₂-fri Extra Buffer, 25 mM MgCl₂
Certifikater
Taq DNA-polymerase, 10X MgCl₂-fri Extra Buffer, 25 mM MgCl₂
1.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 1.000 EE
1 * 1.000 EE - kr2,630.00
kr2,630.00
1 * 1.000 EE
kr2,630.00
Specifikationer:
More
Specifications
Cat. No.
733-1305
Beskrivelse
Taq DNA-polymerase, 10X MgCl₂-fri Extra Buffer, 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Detergent free
Varmstart
No
Koncentration
5 U/µl
Enheder
1000
Størrelse
2 x 0.1 ml + 4 x 1.5 ml
Buffer sammensætning
10x Extra Buffer and 25 mM MgCl2
Detergent free
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1307
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
1.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 1.000 EE
1 * 1.000 EE - kr2,610.00
kr2,610.00
1 * 1.000 EE
kr2,610.00
Specifikationer:
More
Specifications
Cat. No.
733-1307
Beskrivelse
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Detergent free
Varmstart
No
Koncentration
5 U/µl
Enheder
1000
Størrelse
2 x 0.1 ml + 4 x 1.5 ml
Buffer sammensætning
10x Key Buffer and 25 mM MgCl2
Detergent free
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification
733-1823
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
10.000 EU
Log ind for tilgængelighed
Log ind for tilgængelighed
1 * 10.000 EE
1 * 10.000 EE - kr18,100.00
kr18,100.00
1 * 10.000 EE
kr18,100.00
Specifikationer:
More
Specifications
Cat. No.
733-1823
Beskrivelse
Taq DNA-polymerase, 10X Tween-fri Key Buffer (15 mM MgCl₂), 10X triton-fri Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
Format
Enzyme with/without buffer
Funktioner
Detergent free
Varmstart
No
Koncentration
5 U/µl
Enheder
10000
Størrelse
3 x 0.667 ml + 18 x 5 ml
Buffer sammensætning
10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Detergent free
Høj troskab
No
Amplikon størrelse
≤ 5kb
Processivity
Standard
Exonuklease aktivitet
5’→3’
Product overhang
3'A
Opbevaring betingelser
– 20 °C (long term), + 4 °C (6 months)
Ansøgning
Standard amplification