VWR®, TEMPase Hot Start 2× master mix
About this item
TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.
The blue loading dye in Blue TEMPase Hot Start DNA Polymerase Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.
Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR.
Delivery information: TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included in the kit to enable optimisation.
5 Options Available Below
Product Details & Documents
TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.
The blue loading dye in Blue TEMPase Hot Start DNA Polymerase Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.
Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR.
Delivery information: TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included in the kit to enable optimisation.
Documents
Certificates
Search by Lot NumberTDS Blue TEMPase Hot Start Master Mix C 2500 Reactions TDS Blue TEMPase Hot Start Master Mix K 2500 Reactions TDS Multiplex TEMPase 2x Master Mix 2500 Reactions TDS Taq DNA Polymerase, VWR TDS TEMPase Hot Start Master Mix C 2500 Reactions
Datasheet
Versatile VWR PCR enzymes VWR Taq DNA polymerase is more thermo-stable than most people think VWR TEMPase Hot Start DNA polymerase - for robust and reliable PCR
VWR® for PCR
Product Literature
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 733-2582
- 733-1840
- 733-2585
- 733-1841
- 733-2569
- Beskrivelse:
- TEMPase Hot Start 2X Master Mix, med Master Mix K, 1,5 mM MgCl₂
- TEMPase Hot Start 2X Master Mix, med Master Mix C, 1,5 mM MgCl₂
- Blue TEMPase Hot Start 2X Master Mix, med Blue Master Mix K, 1,5 mM MgCl₂
- Blue TEMPase Hot Start 2X Master Mix, med Blue Master Mix C, 1,5 mM MgCl₂
- Multiplex TEMPase Hot Start 2X Master Mix, 1,5 mM MgCl₂ med ekstra MgCl₂
- Format:
- Mastermix incl. dNTPs
- Mastermix incl. dNTPs
- Mastermix incl. dNTPs
- Mastermix incl. dNTPs
- Mastermix incl. dNTPs
- Funktioner:
- Direct loading
- Direct loading
- Varmstart:
- Chemically modified
- Chemically modified
- Chemically modified
- Chemically modified
- Chemically modified
- Koncentration:
- 2x
- 2x
- 2x
- 2x
- 2x
- Enheder:
- 2500 r Mix 2500
- 2500 r Mix 2500
- 2500 r Mix 2500
- 2500 r Mix 2500
- 2500 r Mix 2500
- Størrelse:
- 50 x 1.25 ml
- 50 x 1.25 ml
- 50 x 1.25 ml
- 50 x 1.25 ml
- 50 x 1.25 ml + 3 x 1.5 ml
- Buffer sammensætning:
- Based on Ammonium buffer
- Based on Combination buffer
- Based on Ammonium buffer
- Based on Combination buffer
- 25 mM MgCl2
- Høj troskab:
- No
- No
- No
- No
- No
- Amplikon størrelse:
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- Processivity:
- Standard
- Standard
- Standard
- Standard
- Standard
- Exonuklease aktivitet:
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- Product overhang:
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- Opbevaring betingelser:
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- Ansøgning:
- Multiplex
- Multiplex
- Multiplex
- Multiplex
- Multiplex
Specifications
Product Family Options
Product Information
- BeskrivelsePaknAvailabilityPrice
- TEMPase Hot Start 2X Master Mix, med Master Mix K, 1,5 mM MgCl₂2.500 TestsLog in for availability1 * 1 Each23.840,00 krSpecifications:
More
Specifications
Cat. No.733-2582BeskrivelseTEMPase Hot Start 2X Master Mix, med Master Mix K, 1,5 mM MgCl₂FormatMastermix incl. dNTPsFunktionerVarmstartChemically modifiedKoncentration2xEnheder2500 r Mix 2500Størrelse50 x 1.25 mlBuffer sammensætningBased on Ammonium bufferHøj troskabNoAmplikon størrelse≤ 5kbProcessivityStandardExonuklease aktivitet5’→3’Product overhang3'AOpbevaring betingelser– 20 °C (long term), + 4 °C (6 months)AnsøgningMultiplex - TEMPase Hot Start 2X Master Mix, med Master Mix C, 1,5 mM MgCl₂2.500 TestsLog in for availability1 * 1 Each19.100,00 krSpecifications:
More
Specifications
Cat. No.733-1840BeskrivelseTEMPase Hot Start 2X Master Mix, med Master Mix C, 1,5 mM MgCl₂FormatMastermix incl. dNTPsFunktionerVarmstartChemically modifiedKoncentration2xEnheder2500 r Mix 2500Størrelse50 x 1.25 mlBuffer sammensætningBased on Combination bufferHøj troskabNoAmplikon størrelse≤ 5kbProcessivityStandardExonuklease aktivitet5’→3’Product overhang3'AOpbevaring betingelser– 20 °C (long term), + 4 °C (6 months)AnsøgningMultiplex - Blue TEMPase Hot Start 2X Master Mix, med Blue Master Mix K, 1,5 mM MgCl₂2.500 TestsLog in for availability1 * 1 Each23.840,00 krSpecifications:
More
Specifications
Cat. No.733-2585BeskrivelseBlue TEMPase Hot Start 2X Master Mix, med Blue Master Mix K, 1,5 mM MgCl₂FormatMastermix incl. dNTPsFunktionerDirect loadingVarmstartChemically modifiedKoncentration2xEnheder2500 r Mix 2500Størrelse50 x 1.25 mlBuffer sammensætningBased on Ammonium bufferHøj troskabNoAmplikon størrelse≤ 5kbProcessivityStandardExonuklease aktivitet5’→3’Product overhang3'AOpbevaring betingelser– 20 °C (long term), + 4 °C (6 months)AnsøgningMultiplex - Blue TEMPase Hot Start 2X Master Mix, med Blue Master Mix C, 1,5 mM MgCl₂2.500 TestsLog in for availability1 * 1 Each19.100,00 krSpecifications:
More
Specifications
Cat. No.733-1841BeskrivelseBlue TEMPase Hot Start 2X Master Mix, med Blue Master Mix C, 1,5 mM MgCl₂FormatMastermix incl. dNTPsFunktionerDirect loadingVarmstartChemically modifiedKoncentration2xEnheder2500 r Mix 2500Størrelse50 x 1.25 mlBuffer sammensætningBased on Combination bufferHøj troskabNoAmplikon størrelse≤ 5kbProcessivityStandardExonuklease aktivitet5’→3’Product overhang3'AOpbevaring betingelser– 20 °C (long term), + 4 °C (6 months)AnsøgningMultiplex - Multiplex TEMPase Hot Start 2X Master Mix, 1,5 mM MgCl₂ med ekstra MgCl₂2.500 TestsLog in for availability1 * 1 Each34.900,00 krSpecifications:
More
Specifications
Cat. No.733-2569BeskrivelseMultiplex TEMPase Hot Start 2X Master Mix, 1,5 mM MgCl₂ med ekstra MgCl₂FormatMastermix incl. dNTPsFunktionerVarmstartChemically modifiedKoncentration2xEnheder2500 r Mix 2500Størrelse50 x 1.25 ml + 3 x 1.5 mlBuffer sammensætning25 mM MgCl2Høj troskabNoAmplikon størrelse≤ 5kbProcessivityStandardExonuklease aktivitet5’→3’Product overhang3'AOpbevaring betingelser– 20 °C (long term), + 4 °C (6 months)AnsøgningMultiplex
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck