VWR®, TEMPase Hot Start 2× master mix

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

The blue loading dye in Blue TEMPase Hot Start DNA Polymerase Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.

Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR.

: TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included in the kit to enable optimisation.

Ansøgning:
Multiplex
Format:
Mastermix incl. dNTPs
Høj troskab:
No
Varmstart:
Chemically modified
Storage conditions:
– 20 °C (long term), + 4 °C (6 months)
Amplikon størrelse:
≤ 5kb
Processivity:
Standard
Exonuklease aktivitet:
5’→3’
Product overhang:
3'A
Buffer sammensætning:
Based on Ammonium buffer
Koncentration:
2x
Beskrivelse:
TEMPase Hot Start DNA Polymerase 2X Master Mix A
1.5 mM MgCl2 2500 reactions
Størrelse:
50 x 1.25 ml
Enheder:
2500 r Mix 2500
Funktioner:
Direct loading