High pH reversed-phase kit, Pierce™

Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.

• Easy-to-use with resin provided in single-use spin column format
• Protein identifications increased by ≥50% when compared to unfractionated samples
• Reproducible with elution profiles and fractional resolution varying by less than 20%
• Optimised, robust procedure for maximal protein identification and peptide recovery while minimising fractional overlap
• Compatible with reagents validated with a variety of complex samples, including TMT™-labeled peptides
• Can be detected by mass spectrometry method

This fractionation kit utilises high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.

The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a high pH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.

Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.

Applications include reducing sample complexity to identify targets of interest, conducting systems biology studies and reducing sample complexity to improve quantitation studies.

Leveringsinformation: Includes a high pH buffer (0,1% triethylamine) and 12 spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10 to 100 µg of peptide sample using a microcentrifuge.

Advarsel: For research use only. Not for use in diagnostic procedures.