X-Gal staining kit

X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

• Convenient for all LacZ transfection assays
• Suitable for cultured cells and tissues
• Complete kit provided with substrate and buffers
• Economical

LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, beta-Gal), is very stable, resistant to proteolytic degradation and easily tested.

 : Kit contains fixing buffer (125 ml), 10X PBS (75 ml), staining buffer (125 ml) and 25X stock solution of X-Gal (4 ml).

 : Store at +4 °C except 25X stock solution of X-Gal –20 °C.