SEAP assay kit

SEAP assay kit (Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from transfected cells or tissues.

• Designed to detect SEAP reporter gene expression
• Fast and easy analysis
• Colorimetric-measure with a standard spectrophotometer or an ELISA reader (405 nm)
• Multiple samples can be analysed in low volumes
• Convenient and economical packaging: no tablet, no powder

Alkaline phosphatase cleaves the colourless 4-nitrophenylphosphate (pNPP) substrate to give a yellow p-nitrophenolate product in alkaline solutions. It is commonly used as a reporter gene expression and can be accurately quantified. It is a truncated form of placental alkaline phosphatase (GPI-anchored protein).

 : Kit contains pNPP-containing assay buffer component A (10×1 ml), reaction buffer component B (125 ml), reaction buffer component C (125 µl) and dilution buffer component D (50 ml).

 : Store at +4 °C.