CPRG assay kit

CPRG assay kit is designed to quantify low expression level β-Galactosidase.

• Convenient for all transfection assays
• Suitable for cultured cells and tissues
• Simple, rapid and easy to use
• Economical
• Optimised with stable buffers

The levels of active β-Galactosidase expression can be quickly measured by its catalytic hydrolysis of CPRG (Chlorophenol red-β-D-galactopyranoside), substrate to a dark red product. The high sensitivity of this substrate improves the measurement of β-Galactosidase activity when the reporter gene expression is low (absorbance at 570 to 595 nm).  All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.

LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, β-Gal), is very stable, resistant to proteolytic degradation and easily assayed.

Leveringsinformation: Kit contains lysis buffer (55 ml), standard dillution buffer (55 ml), 10X CPRG (5 ml), substrate buffer (55 ml), stop buffer (55 ml) and beta Gal enzyme (100 µl).

Advarsel: Store all reagents at +4 °C except β-Gal enzyme and CPRG substrate stock solution.