Easy-Titer® IgG assay kits are simple, mix-and-read assays that allow quick and accurate determination of concentrations from 15 to 300 ng/ml of IgG.

• Faster and easier than ELISA
• Sensitive, requires small sample volume
• No special equipment needed
• Highly reproducible results

The assay procedure uses monodispersed polystyrene beads that are coated with anti-IgG antibodies and absorb light at 340 and 405 nm. When the beads are mixed with a sample containing IgG, they aggregate, causing decreased absorption of light and, therefore, low IgG concentrations yield high absorbance values and high IgG concentrations yield low absorbance values. The decrease in absorption is proportional to IgG concentration and a standard curve can be generated to accurately quantify levels of IgG in serum, ascites or cell culture supernatant samples.