NGF Rapid™ ELISA kit: mouse
Om denne vare
The Biosensis NGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed.
This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples.
The purpose of this kit is the in vitro quantitative determination of NGF in samples such as serum, plasma, cell culture supernatants and brain extracts.
Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was <0,1% when assayed at 25 ng/ml in assay buffer, and not detectable when assayed across the mature NGF calibration range.
This kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of NGF present in samples and protein standards.
Please refer to the kit protocol for specific use instructions for each substrate application.
Leveringsinformation: Each kit contains 1 or 2× 96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed instructions.
Advarsel: For research use only. Not for diagnostic or clinical purposes.
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Product Details & Documents
The Biosensis NGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed.
This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples.
The purpose of this kit is the in vitro quantitative determination of NGF in samples such as serum, plasma, cell culture supernatants and brain extracts.
Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was <0,1% when assayed at 25 ng/ml in assay buffer, and not detectable when assayed across the mature NGF calibration range.
This kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of NGF present in samples and protein standards.
Please refer to the kit protocol for specific use instructions for each substrate application.
Leveringsinformation: Each kit contains 1 or 2× 96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed instructions.
Advarsel: For research use only. Not for diagnostic or clinical purposes.
Documents
Specifications for Product Family
Specifikationer
Specifikationer
- Catalog No:
- BSENBEK-2213-1P
- BSENBEK-2213-2P
- Target protein:
- NGF
- NGF
- Primary antibody reactivity:
- Mouse
- Mouse
- Analysetype:
- Sandwich
- Sandwich
- Format:
- Pre-coated
- Pre-coated
- Sensitivitet:
- Typical limit of detection (LOD) for mouse NGF is 8 pg/ml determined as 150% of the blank value
- Typical limit of detection (LOD) for mouse NGF is 8 pg/ml determined as 150% of the blank value
- Detektionsområde:
- 3,9 – 250 pg/ml
- 3,9 – 250 pg/ml
- Krydsreaktivitet:
- The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/ml in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat. Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3,9-250 pg/ml; 0,14-9,2 pmol/l). OD readings for mouse proNGF were indistinguishable from the assay’s blank OD readings.
- The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/ml in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat. Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3,9-250 pg/ml; 0,14-9,2 pmol/l). OD readings for mouse proNGF were indistinguishable from the assay’s blank OD readings.
Specifikationer
Product Family Options
Produktinformation
- PaknTilgængelighedPrice
- BSENBEK-2213-1PNGF Rapid™ ELISA Kit: Mouse, 1×96-well plateNGF Rapid™ ELISA Kit: Mouse, 1×96-well plate96 TestsLog ind for tilgængelighed1 * 1 Each3.180,00 krSpecifikationer:
More
Specifications
Cat. No.BSENBEK-2213-1PTarget proteinNGFPrimary antibody reactivityMouseAnalysetypeSandwichFormatPre-coatedSensitivitetTypical limit of detection (LOD) for mouse NGF is 8 pg/ml determined as 150% of the blank valueDetektionsområde3,9 – 250 pg/mlKrydsreaktivitetThe antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/ml in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat. Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3,9-250 pg/ml; 0,14-9,2 pmol/l). OD readings for mouse proNGF were indistinguishable from the assay’s blank OD readings. - BSENBEK-2213-2PNGF Rapid™ ELISA Kit: Mouse, 2×96-well plateNGF Rapid™ ELISA Kit: Mouse, 2×96-well plate192 TestsLog ind for tilgængelighed1 * 1 Each5.790,00 krSpecifikationer:
More
Specifications
Cat. No.BSENBEK-2213-2PTarget proteinNGFPrimary antibody reactivityMouseAnalysetypeSandwichFormatPre-coatedSensitivitetTypical limit of detection (LOD) for mouse NGF is 8 pg/ml determined as 150% of the blank valueDetektionsområde3,9 – 250 pg/mlKrydsreaktivitetThe antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/ml in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat. Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3,9-250 pg/ml; 0,14-9,2 pmol/l). OD readings for mouse proNGF were indistinguishable from the assay’s blank OD readings.
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