Affinity chromatography columns, GSTrap™ FF
Om denne vare
GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scale-up by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.
The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
4 tilgængelige muligheder nedenfor
Product Details & Documents
GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scale-up by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.
The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
Documents
Cytiva CyDye Licensing Statements Cytiva General Licensing Statements
Additional Product Details
Specifications for Product Family
Specifikationer
- Catalog No:
- 17-5130-02
- 17-5130-01
- 17-5131-01
- 17-5131-02
- Maks. tryk:
- 5 bar
- 5 bar
- 5 bar
- 5 bar
- pH-område:
- 3 - 12
- 3 - 12
- 3 - 12
- 3 - 12
- Opbevaring:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
Specifikationer
Product Family Options
Produktinformation
- KolonnematerialePartikelstørrelseSengens dimensionerKapacitetFlowhastighedStrømningshastighedTilgængelighedPrice
- 17-5130-02GSTrap™ FFSpecifikationer:
More
Specifications
Cat. No.17-5130-02Maks. tryk5 barpH-område3 - 12Opbevaring4 to 30 °C, 20% Ethanol - 17-5130-01GSTrap™ FFSpecifikationer:
More
Specifications
Cat. No.17-5130-01Maks. tryk5 barpH-område3 - 12Opbevaring4 to 30 °C, 20% Ethanol - 17-5131-01GSTrap™ FFGSTrap™ FFPP90 µm16×25 mm1×5 ml<20 ml/min<450 cm/hLog ind for tilgængelighed1 * 1 Items2.690,00 krSpecifikationer:
More
Specifications
Cat. No.17-5131-01Maks. tryk5 barpH-område3 - 12Opbevaring4 to 30 °C, 20% Ethanol - 17-5131-02GSTrap™ FFGSTrap™ FFPP90 µm16×25 mm5×5 ml<20 ml/min<450 cm/hLog ind for tilgængelighed1 * 5 Items10.630,00 krSpecifikationer:
More
Specifications
Cat. No.17-5131-02Maks. tryk5 barpH-område3 - 12Opbevaring4 to 30 °C, 20% Ethanol
Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".
Otherwise, you will receive generic recommendations.
Catalog #: 83639.320
Leverandør: VWR Chemicals
Catalog #: 20864.320
Leverandør: VWR Chemicals
Catalog #: 83640.320
Leverandør: VWR Chemicals
Catalog #: 1.00030.2500
Leverandør: Merck
Catalog #: 31770.294
Leverandør: VWR Chemicals
Catalog #: 83645.320
Leverandør: VWR Chemicals
Catalog #: 1.09439.1000
Leverandør: Merck