Celler

Supplier: Charles River Laboratories Cell Solutions

CD4⁺/CD25⁺ regulatory T cells were isolated from leukapheresis product by CD127 depletion and positive selection using CD25 immunomagnetic beads. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Charles River Laboratories Cell Solutions

Human mononuclear cells are isolated from IRB-consented volunteer donors diagnosed with HIV or AIDS.

Supplier: ProSci Inc.

Brain tissue lysate (0 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Charles River Laboratories Cell Solutions

CD8⁺ cytotoxic T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: ProSci Inc.

Heart tissue lysate (7 day old mouse) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: PROVITRO

Human Adipose-Derived Stem Cells (ADSCs) are extracted from human lipoaspirate tissue obtained during elective liposuction surgeries. These cells are available from both healthy donors and patients. ADSCs exhibit phenotypic and functional characteristics similar to bone marrow-derived mesenchymal stem cells.

Supplier: Charles River Laboratories Cell Solutions

Naive T cells are isolated by depleting CD45RO^₊ cells via immunomagnetic bead separation from a freshly collected leukopheresis product. Naive T cells are phenotypically classified as CD45⁺, CD^₊, and CD45RA^₊.

Supplier: Charles River Laboratories Cell Solutions

Mononuclear cells used for researching the causes, diagnosis, supportive care and treatment of Diabetes Mellitus 2 (DM2), the most common form of diabetes. Human mononuclear cells are collected from human volunteer donors diagnosed with DM2 under IRB approved informed consent by whole blood or leukapheresis using the COBE® Spectra Apheresis System MNC collection protocol.

Supplier: PROVITRO

Bone Marrow CD34⁺ cells, enriched with CD34⁺ markers, are pivotal for haematopoiesis and immune modulation. These unique cells play a crucial role in hematopoietic stem cell transplantation and immune reconstitution. They contribute to the formation of various blood cell lineages, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: PROVITRO

HCASMC - Human coronary artery smooth muscle cells - are obtained from human tissue, in particularly from the coronary artery smooth muscle cells, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation.

Supplier: ProSci Inc.

Lung tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Charles River Laboratories Cell Solutions

CD3⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: Charles River Laboratories Cell Solutions

CD56⁺ NK cells are isolated from mononuclear cells either by positive selection using an antibody that targets the CD56 antigen, or by negative selection using immunomagnetic cell separation procedures.

Supplier: ProSci Inc.

Skin tissue lysate (0 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.