Kromatografi buffere
Kromatografibuffere optimerer proteinoprensning og genvindingsprocedurer. Ved eluering fra den stationære fase ændrer opløsningerne pH-niveau for at svække den elektrostatiske kraft. De salte stoffer genererer og opretholder pH-niveauer til korrekt ionbytning, størrelseseksklusion og affinitet. Ved blot at ændre forholdet mellem syre og salt ændres kromatografibufferens pH tilsvarende. Udviklet til forskellige separationsmetoder leveres de koncentrerede væsker eller krystallinske formater færdiglavede for at reducere forberedelsestiden.
Borate buffer for HPLC, LiChropur™, Supelco®
Supplier: Merck
Borate buffer, 0.127 - 0.140 M
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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®
Supplier: Merck
1M Buffer, also for use in ion-exchange chromatography and electrophoresis.
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Triethylammonium bicarbonate buffer, 1 M, volatile buffer for HPLC, Fluka™
Supplier: Honeywell Chemicals
Triethylammonium bicarbonate buffers are volatile buffer for HPLC.
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Ammonia buffer solution pH10 (20 °C) for complexometry, Fluka™
Supplier: Honeywell Chemicals
Ammonia buffer
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His buffer kit
Supplier: Cytiva
His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.
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#: 20864.320
: VWR Chemicals
#: 83640.320
: VWR Chemicals
#: 31770.294
: VWR Chemicals
#: 83645.320
: VWR Chemicals