Elektroforese Farvestoffer
Klar-til-brug elektroforesebuffere, geler og pulverfarvestoffer sorterer hurtigt prøve-molekyler baseret på størrelse og ladning. Disse reagenser skaber mindre baggrundsfluorescens, som ellers kan påvirke visuelle studier negativt. For at opnå den nødvendige kemiske reaktion skal du vælge farvetypen baseret på miljøforhold og forsøgets mål. Fra det almindeligt anvendte Coomassie Brilliant Blue til det kræftforskningsspecifikke Papanicolaou gør følsomme elektroforesefarvestoffer cellekomponenter synlige for yderligere DNA- og RNA-analyser.
VWR®, Nucleic acid loading dyes
Supplier: VWR Chemicals
Designed to optimise loading nucleic acids in agarose gels, the loading dye imparts colour to the sample to facilitate the loading process and increase the density of the sample to ensure efficient sample distribution into each well. The dye migrates independently from the sample, allowing estimation of the migration of nucleic acids.
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VWR® BANDit™ Protein Stain Blue
Supplier: VWR Chemicals
Blue BANDit is a safe and environmentally friendly alternative to traditional Coomassie® Blue staining.
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VWR® EZ-Vision®, DNA dyes
Supplier: VWR Chemicals
EZ-Vision DNA dye as loading buffer provides a non mutagenic, environmentally friendly alternative to ethidium bromide for instantaneous fluorescent DNA band visualisation.
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VWR®, DNA loading buffers
Supplier: VWR Chemicals
These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.
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VWR® EZ-Vision® Bluelight DNA Dye 10000X
Supplier: VWR Chemicals
EZ-Vision BlueLight is a sensitive, non mutagenic and environmentally safe fluorescent dye designed for gel staining.
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VWR®, Loading dye base
Supplier: VWR Chemicals
This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.
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ApolloGreen Stain-Safe DNA Dye
Supplier: Apollo Scientific
ApolloGreen Stain-Safe DNA dye is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels. It binds to both dsDNA, ssDNA and RNA and emits green fluorescence when bound to DNA and red fluorescence when bound to RNA detectable under UV or Blue light and is documented with same filters as similar green dyes. ApolloGreen Stain-Safe DNA Dye is ideal for DNA extraction from gels for cloning. ApolloGreen Stain-Safe DNA dye is a significantly safer alternative to ethidium bromide. It is same easy to use, 4X as sensitive (under Blue LED) and much more secure. More economical as other green dyes, this stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide.
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Gel loading dye, orange (6X)
Supplier: Thermo Fisher Scientific
Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.
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Gelfarver til nukleinsyrer, GelRed™ og GelGreen™
Supplier: Biotium
GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.
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Electrophoresis gel stain, Reversible Copper Stain™
Supplier: G-Biosciences
The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.
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Electrophoresis gel stain, Reversible Zinc Stain™
Supplier: G-Biosciences
A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.
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BLOT-FastStain™
Supplier: G-Biosciences
BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.
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LabSafe™ GEL blue for staining of polyacrylamide gels
Supplier: G-Biosciences
LabSafe GEL Blue™ is an enhanced protein stain based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualisation.
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Silver stain, FOCUS™ FASTsilver™
Supplier: G-Biosciences
FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.
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Glycoprotein stain
Supplier: G-Biosciences
The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.
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Nucleic acid stains, PAGE GelRed™
Supplier: Biotium
Stains specifically designed for DNA in polyacrylamide gels.
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