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Agarose og Akrylamid

Agarose og Akrylamid

Agarose og akrylamid er de mest almindeligt anvendte elektroforese-geler på grund af deres alsidighed med buffere og evne til at generere reproducerbare resultater. Agarose bruges typisk til horisontale tests for at adskille store DNA-fragmenter, mens akrylamid bruges til vertikale separationer af kortere nukleinsyrer. Gelerne er lette at håndtere og vælges ud fra det ønskede høje opløsningsniveau og prøverne. Koncentrationen eller forholdet mellem agarose og akrylamid bestemmer den samlede arbejdsporøsitet.

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VWR® Acrylamide-​Bis Solution (37,5:1) 30% Aqueous Solution, Ultra Pure Grade

VWR® Acrylamide-​Bis Solution (37,5:1) 30% Aqueous Solution, Ultra Pure Grade

Supplier: VWR Chemicals

Electrophoresis Gels, Acryl/Bis™, Acrylamide-​Bis solution (37.5: 1) 30% aqueous solution, ultra rent

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GelGreen® Agarose LE

Supplier: Biotium

Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic acid gel stain for convenient and safer preparation of precast gels.

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Peqlab peqGOLD, Agarose, Universal

Peqlab peqGOLD, Agarose, Universal

Supplier: VWR Chemicals

High quality standard agarose for routine analysis with broad separation range (0,05 to 50 kbp). Ideal for blotting applications due to high gel strength. Free from RNases, DNases and enzyme inhibitors.

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VWR® Acrylamide and Bis-Acrylamide

VWR® Acrylamide and Bis-Acrylamide

Supplier: VWR Chemicals

An extensive line of acrylamide and bis-acrylamide pre-weighed powder blends, pre-mixed stock solutions and ready to use solutions for customised polyacrylamide gel electrophoresis of proteins and PAGE of nucleic acids.

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Agarose LE, ultra-pure molecular bology grade

Supplier: Biotium

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with GelRed®, GelGreen®, and DNAzure® gel stains.

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VWR®, Agarose RA™

VWR®, Agarose RA™

Supplier: VWR Chemicals

Agarose RA™ is a general application agarose that provides good resolution and is cost effective for high volume users. It was developed specifically for quick checks of PCR products, plasmids preps, screening and cloning.

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VWR® Acryl/Bis™ Acrylamine/Bis-Acrylamine

VWR® Acryl/Bis™ Acrylamine/Bis-Acrylamine

Supplier: VWR Chemicals

A 40% (w/v) solution of ultra pure acrylamide (38,96%) and bis-acrylamide (1,04%) at a final ratio of 37.5:1.

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VWR®, Agarose I™

VWR®, Agarose I™

Supplier: VWR Chemicals

Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid analytical/preparative applications (250 bp to 22 kb).

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VWR® Acrylamide-Bis (29:1), 30% Aqueous Solution, Ultra-pure Grade

VWR® Acrylamide-Bis (29:1), 30% Aqueous Solution, Ultra-pure Grade

Supplier: VWR Chemicals

Electrophoresis Gels, Acryl/Bis™, Acrylamide-Bis (29: 1), 30% aqueous solution, ultra rent

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Agarose II, low melting point, Pierce™

Supplier: Thermo Fisher Scientific

Agarose II (low melt) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels that require a lower melting temperature.

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Agarose, MetaPhor™

Agarose, MetaPhor™

Supplier: LONZA

Intermediate melting point agarose ideal for resolution of DNA fragments, PCR and RT-PCR products, differing in size by 2%, in the range of 20 to 800 bp, by submarine gel electrophoresis.

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Melt & Pour UltraSpec-Agarose™

Supplier: EDVOTEK

0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.

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Agarose, IsoGel™

Agarose, IsoGel™

Supplier: LONZA

IsoGel™ Agarose is a highly purified agarose specifically manufactured for isoelectric focusing (IEF). It has no measurable EEO due to a manufacturing process which minimises fixed anions and mobile cations. IsoGel™ Agarose IEF Plates are sufficiently rigid to be cast in vertical tubes in addition to vertically moulded and horizontally open cast thin gels. Gels cast on GelBond® film dry down quickly and easily to transparent permanent records.

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Agarose, SeaKem® HEEO

Agarose, SeaKem® HEEO

Supplier: LONZA

SeaKem® HEEO Agarose is a very high EEO agarose that is useful in applications requiring significant cathodal migration, such as IEP of IgG and IgM. It can also be used to blend with a lower EEO agarose to achieve a specific EEO value.

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Agarose, SeaKem® Gold

Agarose, SeaKem® Gold

Supplier: LONZA

SeaKem® is a high gel strength, low EEO, standard gelling temperature agarose perfect for electrophoresis and other molecular biology applications. This Genetic Technology Grade™ (GTG) Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE).

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Agarose, SeaPrep™

Supplier: LONZA

Ultra-soft agarose, ideal for high efficiency hybridoma cloning and expansion of cDNA libraries.

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