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Protein Oprensningsreagenser

Protein Oprensningsreagenser

Proteinpurrifikationsreagenser anvendes til at isolere et eller flere proteiner fra en kompleks blanding, typisk celler, væv eller hele organismer. Denne proces er afgørende for karakterisering af funktion og struktur samt for at identificere interaktioner for det ønskede protein. Under de mange trin hjælper proteinpurifikationsreagenser med cellelyse, adskillelse af de opløselige proteinkomponenter fra celleaffald og til sidst adskillelsen af det ønskede protein fra produkt- og procesrelaterede urenheder.

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Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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Recombinant protein L, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein A, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein A/G, Pierce™

Recombinant protein A/G, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Maleimide-activated protein, NeutrAvidin™ Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Supplier: Thermo Fisher Scientific

The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Pierce™ Protein A

Pierce™ Protein A

Supplier: Thermo Fisher Scientific

Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein L, Pierce™

Recombinant protein L, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein L is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of mouse and human antibodies in immunoassays and antibody purification protocols.

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ReadiPrep™ Nickel NTA Magnetic Beads

Supplier: AAT Bioquest

ReadiPrep™ Nickel NTA magnetic beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged proteins.

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Immobilised ficin (agarose resin), Pierce™

Immobilised ficin (agarose resin), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Iminobiotin agarose, Pierce™

Iminobiotin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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Immobilised jacalin agarose, Pierce™

Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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Immobilised TCEP disulphide reducing gel, Pierce™

Immobilised TCEP disulphide reducing gel, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.

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PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™

PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

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