Nukleinsyrereagenser
Nukleinsyrereagenser danner grundlaget for genetisk analyse og molekylærbiologi og spiller en central rolle fra kloning til genekspressionsstudier. Udforsk vores sortiment med højpræcisionsenzymer til end-point PCR, avancerede reagenser til isothermal amplifikation og komplette kits til nukleinsyreoprensning. For præcision i kvantitative analyser kan du finde vores udvalg af qPCR- og RT-qPCR-kits, optimeret til uovertruffen nøjagtighed. Vores portefølje omfatter også reagenser til next-generation sekventering, der tilbyder banebrydende løsninger til genomsekventering og fremskridt inden for personlig medicin.
Mitochondria/Cytosol Fractionation Kit, Abcam
Supplier: Abcam
Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays
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Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®
Supplier: OMEGA BIO-TEK
By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.
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qPCR master mixes, Fast Plus EvaGreen®
Supplier: Biotium
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
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T7 RNA polymerase
Supplier: Thermo Fisher Scientific
T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.
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Restriction enzymes, NcoI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Montage® SEQ96 Sequencing Reaction Cleanup Kits
Supplier: Merck
The Montage® SEQ96 sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.
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Restriction enzymes, NotI, 50 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
GGCCGC sites and cuts best at 37 °C in O buffer.
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TriLink® Engineered RNase Inhibitor
Supplier: TriLink BioTechnologies
Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.
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PerfeCTa® PreAmp 5X SuperMix
Supplier: Quantabio
PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
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Viral RNA Xpress kit, Mag-Bind®
Supplier: OMEGA BIO-TEK
Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.
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Taq DNA polymerase, 1000 U
Supplier: Genscript
Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.
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TriLink® Uridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.
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AccuStart™ II PCR ToughMix®
Supplier: Quantabio
AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.
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E.Z.N.A.® Endo-Free plasmid kits
Supplier: OMEGA BIO-TEK
Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.
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E.Z.N.A.® Plant & Fungal DNA Kit
Supplier: OMEGA BIO-TEK
Isolate DNA from plant or fungal samples using spin columns.
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Bacterial DNA kit, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
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