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Elektroforese buffere

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

Immunoassay Blocking (BSA Free)

Immunoassay Blocking (BSA Free)

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

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SDS-Page running gel buffers, 10X concentrate

SDS-Page running gel buffers, 10X concentrate

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

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PBS tablets

PBS tablets

Supplier: PanReac AppliChem

Tablets for preparation of 100 ml phosphate buffered saline (PBS).

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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Supplier: Rockland Immunochemicals

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

    
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Tris-Glycine buffer

Supplier: EDVOTEK

For vertical polyacrylamide gel electrophoresis.

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Western Blot Transfer buffer, 10X, methanol-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Pierce™

Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Pierce™

Supplier: Thermo Fisher Scientific

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

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Blocker™ Blocking Buffer, BLOTTO in TBS

Blocker™ Blocking Buffer, BLOTTO in TBS

Supplier: Thermo Fisher Scientific

Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3

Supplier: PanReac AppliChem

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3

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TAE buffer, 50X

Supplier: EDVOTEK

This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.

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Gel loading solution, 10X

Supplier: EDVOTEK

1 ml concentrate for 200 samples.

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Wash buffers, femto TBST™ and femto PBST™

Wash buffers, femto TBST™ and femto PBST™

Supplier: G-Biosciences

The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.

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VHB DNA Washing Buffer

Supplier: OMEGA BIO-TEK

VHB buffer must be diluted with ethanol before use.

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Pierce™ Blocking Buffers, Protein Free

Pierce™ Blocking Buffers, Protein Free

Supplier: Thermo Fisher Scientific

These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.

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Wash buffer, Pierce™ Fast Wash Buffer

Wash buffer, Pierce™ Fast Wash Buffer

Supplier: Thermo Fisher Scientific

Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.

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Tris-Borate-EDTA (TBE) buffer, 10X, Molecular biology grade

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Blocking solution, BLOK™ BSA

Blocking solution, BLOK™ BSA

Supplier: G-Biosciences

BLOK™ BSA is a 10% BSA protein blocking solution.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: PanReac AppliChem

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Blocking buffer, Blocker™ BSA (10X) in PBS or TBS

Blocking buffer, Blocker™ BSA (10X) in PBS or TBS

Supplier: Thermo Fisher Scientific

Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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Native sample loading buffer, 2X

Supplier: G-Biosciences

Loading Buffer, Native sample loading buffer (2X)

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Pierce™ Buffer, PBS Solution 20X Concentrate (Phosphate Buffered Saline)

Pierce™ Buffer, PBS Solution 20X Concentrate (Phosphate Buffered Saline)

Supplier: Thermo Fisher Scientific

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

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Blocking buffer, fast blocking, Pierce™

Blocking buffer, fast blocking, Pierce™

Supplier: Thermo Fisher Scientific

This buffer effectively and reliably blocks Western blots in just five minutes to provide low background results and speed up traditional blotting protocols.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: OMEGA BIO-TEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Pierce™ BupH™ Wash Buffers, Dry Blend Pack

Pierce™ BupH™ Wash Buffers, Dry Blend Pack

Supplier: Thermo Fisher Scientific

BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.

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Blocking solution, BLOK™ Casein

Blocking solution, BLOK™ Casein

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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Wash buffer, Pierce™

Wash buffer, Pierce™

Supplier: Thermo Fisher Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)

Pierce™ Lane Marker Non-reducing Sample Buffer (5X)

Supplier: Thermo Fisher Scientific

Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

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