Overførselsmembraner
Meget følsomme transfermembraner anvender kapillærvirkning til at adskille DNA-, RNA- eller proteinfragmenter til detektion. De fungerer godt med de geler og buffere, der kræves til overførsels- og immobiliseringstrin, og er holdbare nok til at kunne gennemgå flere stripping-, reprobing- og hybridiseringscyklusser. De højbindende skiver, ark og ruller fås i forskellige porestørrelser og ladninger, så de passer til alle blotting-teknikker inden for molekylærbiologi og genetisk forskning.
Millipore® Immobilon®-NC Hydrophilic Transfer Membrane
Supplier: Merck
Triton-free, mixed cellulose esters transfer membrane.
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Millipore® Immobilon®-FL NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
Hydrophobic PVDF transfer membrane for western blotting using fluorescent, chemifluorescence, chromogenic, or chemiluminescence.
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Immobilon®-P Blotting Sandwich Transfer Membranes
Supplier: Merck
Immobilon®-P, Immobilon-P blotting sandwich transfer membranes, 7×8,4 cm
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Millipore® Immobilon®-E NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
The first PVDF transfer membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
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Millipore® Immobilon®-NC NOW Bundle Hydrophilic Mixed Cellulose Esters Transfer Membrane
Supplier: Merck
Triton-free, mixed cellulose esters transfer membrane, includes 1 dispenser.
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Overførselsmembraner, Biodyne®
Supplier: Cytiva (Formerly Pall Lab)
Biodyne® Transfer membraner har høj følsomhed og lav baggrund for forbedret detektion og opløsning. De er ideelle til detektion af nukleinsyre.
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Millipore® Immobilon®-PSQ NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
PVDF transfer membrane hydrophobic for western blotting using chemiluminescence, chromogenic or radioactive detection methods.
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Nitrocellulose Transfer Membrane- 0.22 µm, Abcam
Supplier: Abcam
High quality 0.22 um nitrocellulose membranes that are more durable than standard nitrocellulose membranes. ab133413 yields clearer blots as the membrane is less prone to distortion.
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Millipore® Immobilon®-NC Nitrocellulose Transfer Membranes
Supplier: Merck
Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth
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Low Fluorescence Western Membrane (PVDF), Abcam
Supplier: Abcam
Pre-cut membranes (10 sheets, 7 x 9 cm), compatible with gels 10 x 10 cm and smaller.
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Nitrocellulose Transfer Membrane- 0.45 µm, Abcam
Supplier: Abcam
High quality 0.45 um nitrocellulose membranes that are more durable than standard nitrocellulose membranes. ab133412 yields clearer blots as the membrane is less prone to distortion.
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Millipore® Immobilon®-P NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
Hydrophobic immobilon-P PVDF transfer membrane for western blotting using chemiluminescence, chromogenic or radioactive detection methods.
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Millipore® Immobilon® PVDF Transfer Membranes
Supplier: Merck
These Immobilon® PVDF membranes, each optimised for different protein blotting applications.
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Blotting membranes, nitrocellulose
Supplier: Thermo Fisher Scientific
Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.
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Pierce™, Biodyne™ A Blotting Membranes, Nylon
Supplier: Thermo Fisher Scientific
Biodyne™ A nylon membranes are sheets of unmodified nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ A nylon membrane is manufactured with a matrix pore size of 0,45 micron. It contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules.
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Transfer membranes, PVDF, Pierce™
Supplier: Thermo Fisher Scientific
Transfer membranes made of high quality PVDF, which provides high binding capacity for proteins and nucleic acids.
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