Proteinassays
Præcis kvantificering af den totale proteinkoncentration er nøglen til de fleste eksperimenter og er ofte nødvendig for isolering, separation og analyse af proteiner ved biokemiske metoder. Når det er nødvendigt at bestemme den totale proteinkoncentration i en prøve, er en af de første faktorer at vælge en egnet proteinassay-metode. Dette valg er typisk baseret på metodens kompatibilitet med de prøver, der skal analyseres. Hver metode har sine egne fordele og ulemper.
VWR® Protein Precipitation Kit
Supplier: VWR Chemicals
The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
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Bicinchoninic acid disodium salt hydrate
Supplier: SIGMA ALDRICH MICROSCOPY
Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.
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Thermal shift protein stability kits, GloMelt™
Supplier: Biotium
Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
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Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences
Supplier: Tonbo Biosciences
Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
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Protein assay kits, RED 660™
Supplier: G-Biosciences
RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.
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Protein carbonyl colorimetric assay kit
Supplier: Cayman Chemical
The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
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Bicinchoninic acid (BCA) reducing agent compatible protein Assay
Supplier: G-Biosciences
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.
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Conjugate stabilisers
Supplier: Rockland Immunochemicals
Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.
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Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay
Supplier: G-Biosciences
A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
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Protein Digestibility Assay Kit
Supplier: MEGAZYME
The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method).
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Glycogen Assay Kit
Supplier: Cayman Chemical
A simple and sensitive tool for assaying glycogen from tissue.
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Bicinchoninic acid (BCA) protein assay
Supplier: G-Biosciences
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.
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Micro Bicinchoninic Acid (BCA) protein assay
Supplier: G-Biosciences
The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).
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Compatible Lowry protein assay
Supplier: G-Biosciences
These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.
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L-Lactate Assay Kit
Supplier: Cayman Chemical
A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
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VWR®, Bradford protein assay, proteomics grade
Supplier: VWR Chemicals
The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.
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