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Du søgte efter: Cellulær Assays

Cellulær Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Cholesterol cell-based detection assay kit

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

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Calcium assay kits, FLIPR® Calcium 4 assay kit

Calcium assay kits, FLIPR® Calcium 4 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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Calcium assay kit

Supplier: Cayman Chemical

For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.

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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Supplier: Molecular Devices

SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.

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Steady membrane staining kits, CellBrite™

Steady membrane staining kits, CellBrite™

Supplier: Biotium

Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

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Flow cytometry fixation/permeabilisation kit

Flow cytometry fixation/permeabilisation kit

Supplier: Biotium

Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.

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CFDA SE Cell proliferation assay kit

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

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Cyclic-AMP fluorescent assay kits, CatchPoint™

Cyclic-AMP fluorescent assay kits, CatchPoint™

Supplier: Molecular Devices

CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium

RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium

Supplier: Biotium

RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

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DNA quantitation, SpectraMax® Quant™ dsDNA assay kits

DNA quantitation, SpectraMax® Quant™ dsDNA assay kits

Supplier: Molecular Devices

SpectraMax® Quant™ dsDNA Assay kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Firefly luciferase assay kit (Lyophilised)

Supplier: Biotium

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

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Bacterial viability and gram stain kit

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

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Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors*

Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors*

Supplier: AAT BIOQUEST

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

Supplier: AAT BIOQUEST

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability and proliferation.

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Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit*Optimized for Flow Cytometry*

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit*Optimized for Flow Cytometry*

Supplier: AAT BIOQUEST

Mitochondria are major producers of cellular superoxide.

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MycoLight™ Green JJ99 *5 mM in DMSO*

MycoLight™ Green JJ99 *5 mM in DMSO*

Supplier: AAT BIOQUEST

MycoLight™ Green JJ99 stain is an excellent green-fluorescent nuclear and chromosome counterstain that is permeant to both prokaryotic and eukaryotic cell membranes.

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PUR-1

PUR-1

Supplier: AAT BIOQUEST

PUR-1 is essentially non-fluorescent in the absence of nucleic acids.

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Annexin V-Cy7 Conjugate

Annexin V-Cy7 Conjugate

Supplier: AAT BIOQUEST

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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CytoTell™ Green

CytoTell™ Green

Supplier: AAT BIOQUEST

Flow cytometry combined with fluorescence staining is a powerful tool to analyse heterogeneous cell populations.

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MESG *Phosphate Assay Reagent*

MESG *Phosphate Assay Reagent*

Supplier: AAT BIOQUEST

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

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ReadiUse™ 6-Color Human TBNK Antibody Kit

ReadiUse™ 6-Color Human TBNK Antibody Kit

Supplier: AAT BIOQUEST

Each TBNK dried reagent contains a cocktail of fluorescently-labeled monoclonal antibodies directed against human CD3, CD4, CD8, CD16, C19, CD45, and CD56.

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Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit

Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit

Supplier: AAT BIOQUEST

Provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry-based assay.

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Annexin V, TRITC Labeled

Annexin V, TRITC Labeled

Supplier: AAT BIOQUEST

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Red Fluorescence Optimised for Flow Cytometry*

Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Red Fluorescence Optimised for Flow Cytometry*

Supplier: AAT BIOQUEST

Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes.

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Annexin V, XFD350 Conjugate

Annexin V, XFD350 Conjugate

Supplier: AAT BIOQUEST

XFD350 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 350 (Alexa Fluor® is the trademark of ThermoFisher).

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MitoLite™ Green FM

MitoLite™ Green FM

Supplier: AAT BIOQUEST

MitoLite™ Green FM is the same molecule to the MitoTracker Green FM (M7514, ThermoFisher).

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Cell Navigator® Cell Plasma Membrane Staining Kit *Orange Fluorescence*

Cell Navigator® Cell Plasma Membrane Staining Kit *Orange Fluorescence*

Supplier: AAT BIOQUEST

The Cell Navigator® Plasma membrane stain kit provides a fast and uniform labelling of the plasma membrane without the cell-type differences exhibited by lectins.

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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

The Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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