Du søgte efter: Cellulær Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Glucose (HK) assay kit
Supplier: Merck
The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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ExoBrite™ WGA EV Staining Kits
Supplier: Biotium
ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.
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Calcium assay kits, Fura-2 QBT™ Calcium kit
Supplier: Molecular Devices
The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.
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ViaFluor® 647 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with far-red fluorescence.
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ViaFluor® 405 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with blue fluorescence.
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Solutions colorantes, Ghost Dye™
Supplier: Tonbo Biosciences
Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.
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PPARα transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARα DNA binding activity.
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Cyclic-GMP fluorescent assay kits, CatchPoint™
Supplier: Molecular Devices
CatchPoint™ cGMP Fluorescent Assay Kits measure cGMP levels via a competitive immunoassay format. The second messenger cyclic GMP (cGMP) modulates cellular processes, including smooth muscle relaxation, kidney function, and inflammation, by activating effectors such as protein kinases, cGMP-dependent phosphodiesterases, and cGMP-gated ion channels.
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Reporter assay kits, SpectraMax® Glo Steady-Luc™
Supplier: Molecular Devices
The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.
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Live bacterial gram stain kit
Supplier: Biotium
Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Cytoplasmic membrane labelling kits, CellBrite™
Supplier: Biotium
CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.
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TUNEL assay kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.
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S-Glutathionylated Protein Detection Kit
Supplier: Cayman Chemical
A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.
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Epigenetics Screening Library
Supplier: Cayman Chemical
For screening a variety of epigenetic compounds.
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Neutrophil/Monocyte Respiratory Burst Assay Kit
Supplier: Cayman Chemical
Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
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Apoptosis and necrosis assay kit, NucView™488 RedDot™2
Supplier: Biotium
This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.
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Apoptosis inducer set
Supplier: G-Biosciences
Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.
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HIF-1α transcription factor assay Kit
Supplier: Cayman Chemical
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.
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p53 transcription factor assay kit
Supplier: Cayman Chemical
96-Well assay for measurement of p53.
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PPARδ transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARδ DNA binding activity.
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CF® Dye Dextran 3500 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.
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Cell Viability Reagent Kits for Beckman Coulter Vi-CELL® systems
Supplier: CELL VIABILITY
The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.
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qScript® ultra flex kits
Supplier: Quantabio
Faster, longer, tougher next gen cDNA synthesis with flexible priming.
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Steady membrane staining kits, CellBrite™
Supplier: Biotium
Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.
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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit
Supplier: Molecular Devices
SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium
Supplier: Biotium
RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.