Cellulær Assays
Celleassays har altid været et stærkt værktøj i forskningslaboratoriet. De er utroligt alsidige og kan designes til at måle stort set enhver cellulær eller biokemisk funktion. Følsom detektion af fluorescerende prober hjælper med at evaluere cellesignalering og celledødssignaleringsveje, hvilket er nyttigt til hurtig kvantificering af cellelevedygtighed. Celleassays kan bruges til at måle virkningerne af forskellige behandlinger på cellelevedygtighed og til at evaluere toksiske effekter medieret gennem en række mekanismer.
Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Cyclic-GMP fluorescent assay kits, CatchPoint™
Supplier: Molecular Devices
CatchPoint™ cGMP Fluorescent Assay Kits measure cGMP levels via a competitive immunoassay format. The second messenger cyclic GMP (cGMP) modulates cellular processes, including smooth muscle relaxation, kidney function, and inflammation, by activating effectors such as protein kinases, cGMP-dependent phosphodiesterases, and cGMP-gated ion channels.
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Live bacterial gram stain kit
Supplier: Biotium
Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.
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Reporter assay kits, SpectraMax® Glo Steady-Luc™
Supplier: Molecular Devices
The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.
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PPARα transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARα DNA binding activity.
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ExoBrite™ WGA EV Staining Kits
Supplier: Biotium
ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.
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Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81), Abcam
Supplier: Abcam
Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.
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Fatty Acid Uptake Assay Kit Abcam
Supplier: Abcam
Fatty Acid Uptake Assay Kit (ab287857) enables rapid determination of real-time fatty acid uptake kinetics in live cells expressing fatty acid transporter proteins.
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2-NBDG Glucose Uptake Assay Kit Abcam
Supplier: Abcam
2-NBDG Glucose Uptake Assay Kit ab287845 uses the fluorescent glucose analog 2-NBDG to quantify the rate of cellular glucose uptake. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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LipidLaunch™ BODIPY SM-102 LNP Kit (Loadable)
Supplier: Cayman Chemical
Cayman’s LipidLaunch™ BODIPY SM-102 LNP kit (loadable) provides cargo-ready, empty, BODIPY-labelled SM-102 LNPs. LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. Also demonstrate very low toxicity compared to traditional lipid-based transfection methods.
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Cell Viability Reagent Kits for Beckman Coulter Vi-CELL® systems
Supplier: CELL VIABILITY
The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.
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qScript® ultra flex kits
Supplier: Quantabio
Faster, longer, tougher next gen cDNA synthesis with flexible priming.
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CFDA SE Cell proliferation assay kit
Supplier: Biotium
Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.
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DNA quantitation, SpectraMax® Quant™ dsDNA assay kits
Supplier: Molecular Devices
SpectraMax® Quant™ dsDNA Assay kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations.
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Steady membrane staining kits, CellBrite™
Supplier: Biotium
Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.
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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium
Supplier: Biotium
RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.
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