Cellulær Assays
Celleassays har altid været et stærkt værktøj i forskningslaboratoriet. De er utroligt alsidige og kan designes til at måle stort set enhver cellulær eller biokemisk funktion. Følsom detektion af fluorescerende prober hjælper med at evaluere cellesignalering og celledødssignaleringsveje, hvilket er nyttigt til hurtig kvantificering af cellelevedygtighed. Celleassays kan bruges til at måle virkningerne af forskellige behandlinger på cellelevedygtighed og til at evaluere toksiske effekter medieret gennem en række mekanismer.
Cell proliferation assay kit, WST-1
Supplier: Cayman Chemical
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.
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Potassium assay kits, FLIPR®
Supplier: Molecular Devices
The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.
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CF® Dye Dextran 150000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium
Supplier: Biotium
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.
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Uric acid assay kit
Supplier: Cayman Chemical
Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.
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Nitric oxide reagents and kits
Supplier: Biotium
Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.
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SREBP-2 transcription factor assay kit
Supplier: Cayman Chemical
96 well assay for measurement of SREBP-2.
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Prostaglandin Screening Library I
Supplier: Cayman Chemical
For screening of PGF analogs.
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ROS Detection Cell-Based Assay Kit (DHE)
Supplier: Cayman Chemical
Measure superoxide and hydrogen peroxide levels in living cells.
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Solutions colorantes, Ghost Dye™
Supplier: Tonbo Biosciences
Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.
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ViaFluor® 647 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with far-red fluorescence.
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ViaFluor® 405 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with blue fluorescence.
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Calcium assay kits, Fura-2 QBT™ Calcium kit
Supplier: Molecular Devices
The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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