Proteinekstraktions

Supplier: G-Biosciences

FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

Supplier: G-Biosciences

Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Supplier: G-Biosciences

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

Supplier: G-Biosciences

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

Supplier: Abcam

Protein Extraction Kit (ab270054) is for the solubilization of proteins for a variety of proteomics applications.

Supplier: Biotium

AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).

Supplier: G-Biosciences

Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

Supplier: Thermo Fisher Scientific

Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

Supplier: Abcam

Thrombin cleavage kit efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence. - Suitable for recombinant protein purification and tag removal - Contains sufficient active thrombin enzyme to cleave up to 5 mg of the target protein.

Supplier: Thermo Fisher Scientific

Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

Supplier: Abcam

Abcam's Plant Chromatin Extraction Kit (ab156906) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complexes from plants in a simple and rapid format.

Supplier: G-Biosciences

Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Supplier: Thermo Fisher Scientific

Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

Supplier: Abcam

ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.

Supplier: Thermo Fisher Scientific

Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.