Buffere
Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.
Stacking buffer (4X)
Supplier: Thermo Fisher Scientific
Elektroforesebuffer, Stacking Buffer, 4X
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
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Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot
Supplier: Thermo Fisher Scientific
Washing buffer, Phosphate-buffered saline (PBS, 10X)
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G-Biosciences Acetate Buffer
Supplier: G-Biosciences
Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.
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His·Bind® buffer kit, Novagen®
Supplier: Merck Millipore (Novagen)
The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.
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Buffer, ACES, 0,5 M solution
Supplier: Thermo Fisher Scientific
ACES, 0,5M buffer solution, pH 7,0, Colourless, Liquid
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TE buffer, 1X, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
Use directly to re-suspend and/or dilute purified DNA or RNA.
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TAE buffer, 10X solution, molecular biology grade, ultrapure
Supplier: Thermo Fisher Scientific
10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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BES, 0.5 M buffer solutions
Supplier: Thermo Fisher Scientific
BES buffer solution, 0,5 M, pH-værdi: 6,0, Colourless, Liquid
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20X SSPE, liquid, Corning®
Supplier: Corning
20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.
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TEA, 0,2 M buffer soln., pH 8,0
Supplier: Thermo Fisher Scientific
TEA buffer solution, 0,2 M, pH-værdi: 8,0, Colourless, Odourless, Liquid
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Phosphate, 0,2 M buffer solution, pH 6,8
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,2 M, pH-værdi: 6,8, Liquid
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GST Buffer Kit
Supplier: Cytiva
GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.
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