Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

Buffer, PBS tablets, pH 7,4, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X)

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Buffer, ACES, 0,5 M solution

Supplier: Thermo Fisher Scientific

ACES, 0,5M buffer solution, pH 7,0, Colourless, Liquid

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TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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Buffers, MES, 1,0 M solutions

Supplier: Thermo Fisher Scientific

MES, buffer solution, 1,0 M, pH-værdi: 5,5, Liquid

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Phosphate, 0,5M buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH-værdi: 6,5, Liquid

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HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

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TRIS-buffered saline (TBS, 10X) pH 7,6

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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Stacking buffer (4X)

Supplier: Thermo Fisher Scientific

Elektroforesebuffer, Stacking Buffer, 4X

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Phosphate, 0,2 M buffer solution, pH 7,6

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH-værdi: 7,6, Liquid

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Tween 20 washing buffer, 0,5% in PBS (10X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 0,5% in PBS (10X)

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His·Bind® buffer kit, Novagen®

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.