Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Denaturing lysis buffer

Supplier: Thermo Fisher Scientific

Denaturing lysis buffer, Thermo Fisher Scientific

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Phosphate buffer solution, pH 7,2 ( 25 °C), Fluka™

Supplier: Honeywell Chemicals

Phosphate buffer solution, pH-værdi: 7,20

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Buffer, PBS tablets, pH 7,4, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Buffer, triethylammonium acetate, 1 M solution, pH 7,0, Calbiochem®, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

This buffer solution is used for the purification of chemically synthesised oligonucleotides by HPLC.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffers, MES, 1,0 M solutions

Supplier: Thermo Fisher Scientific

MES, buffer solution, 1,0 M, pH-værdi: 7.5, Liquid

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Tween 20 washing buffer, 0,5% in PBS (10X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 0,5% in PBS (10X)

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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

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Nitrocellulose stripping buffer (10X)

Supplier: Thermo Fisher Scientific

Elektroforesebuffer, Nitrocellulose Stripping Buffer, 10X

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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

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TRIS-buffered saline (TBS, 10X) pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

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Phosphate, 0,5M buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH-værdi: 6,5, Liquid

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.