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Buffere

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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Alkaline running buffer solution

Supplier: Thermo Fisher Scientific

Running Buffer, Alkaline running buffer solution

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Phosphate, 0,5M buffer solution, pH 7,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH-værdi: 7,5, Liquid

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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MOPS-EDTA-sodium acetate buffer (MESA)

Supplier: Thermo Fisher Scientific

MOPS-EDTA-sodium acetate buffer (MESA), Liquid

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TRIS-buffered saline (TBS, 10X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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Hybridisation buffers, formamide-free, HYB-LINK™

Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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Buffer, HEPES solution (1 mol/L), ULTROL Grade, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

HEPES buffer solution (1 mol/l)

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Buffer, TRIS solution pH 7,4 (0,1 mol/L), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

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Buffers, sodium phosphate, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Sodium phosphate, buffer solution, 0,5 M, pH-værdi: 9,5, Liquid

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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

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Buffer solutions, colour coded, Fluka™

Supplier: Honeywell Chemicals

These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.

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Buffers, tricine, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Tricine, 0,5M buffer solution, pH-værdi: 7,5, Colourless, Liquid

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Glycine, 0.2M buffer soln., pH 3.0

Supplier: Thermo Fisher Scientific

Glycine buffer solution, 0,2 M, pH-værdi: 3,0, Odourless, Liquid

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