Buffere
Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.
ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT Bioquest
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
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ReadiUse™ Universal Kinase Reaction Stopping Buffer
Supplier: AAT Bioquest
ReadiUse™ Universal Kinase Reaction Stopping Buffer is an aqueous solution that effectively chelates divalent metal ions.
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ReadiUse™ PCR Reaction Buffer
Supplier: AAT Bioquest
This ready to use PCR buffer contains several components essential for the PCR reaction.
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PBS Tablet (Phosphate Buffered Saline Tablet)
Supplier: AAT Bioquest
Each PBS tablet prepares 100 mL of a 1X PBS solution containing 137 mM sodium chloride, 10 mM phosphate buffer, and 2.7 mM potassium chloride (pH 7.4).
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10X Citrate Buffer, pH 6,0
Supplier: AAT Bioquest
A citrate buffer is an aqueous solution composed of a weak acid (citric acid) and its conjugate base (sodium citrate).
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CytoWatch™ Multiplexing Buffer for Flow Cytometry, 20X
Supplier: AAT Bioquest
CytoWatch™ Multiplexing Buffer is a buffer optimised for the multicolor flow cytometric analysis of cells.
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ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry
Supplier: AAT Bioquest
The ReadiUse™ Annexin Binding Buffer *Optimised for Imaging and Flow Cytometry* is an isotonic buffer designed to promote the labeling of apoptotic cells with annexin V conjugates.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Buffer, HEPES solution (1 mol/L), ULTROL Grade, Millipore®
Supplier: Merck Millipore (Calbiochem)
HEPES buffer solution (1 mol/l)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Buffer, triethylammonium acetate, 1 M solution, pH 7,0, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
This buffer solution is used for the purification of chemically synthesised oligonucleotides by HPLC.
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Buffer, TRIS solution pH 7,4 (0,1 mol/L), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)
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His·Bind® buffer kit, Novagen®
Supplier: Merck Millipore (Novagen)
The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.
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Proteinekstraktionsreagenser, BugBuster® og BugBuster® plus Benzonase®, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® proteinekstraktionsreagens er formuleret til nænsom ødelæggelse af cellevæggen i E. coli, hvilket frigører opløseligt protein. Et simpelt, hurtigt og økonomisk alternativ til mekaniske metoder som French Press eller ultralydslysering. Til frigivelse af udtrykt målprotein i præparation til oprensning eller andre opgaver. Formuleringen benytter sig af en detergentblanding som er i stand til at perforere cellevæggen uden af denaturere det opløselige protein.
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Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®
Supplier: Merck Millipore (Novagen)
Loading Buffer, 4X SDS sample buffer
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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