Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

EPPS, 0,2 M buffer solutions

Supplier: Thermo Fisher Scientific

EPPS buffer solution, 0,2 mol/L, pH-værdi: 8,5, Liquid

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Ammonia buffer solution pH10 (20 °C) for complexometry, Fluka™

Supplier: Honeywell Chemicals

Ammonia buffer

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NP-40 lysis buffer with glycerol (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

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Imidazole-buffered saline (5X)

Supplier: Thermo Fisher Scientific

Washing buffer, Imidazole-buffered saline (5X)

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NP-40 lysis buffer

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, Thermo Fisher Scientific

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PIPES-buffered saline (5X), pH 6,5

Supplier: Thermo Fisher Scientific

PIPES-buffered saline, 5X, pH-værdi: 6,5, Colourless, Liquid

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Buffers, MOPS, 0,5 M solutions

Supplier: Thermo Fisher Scientific

MOPS buffer solution, 0,5 mol/L, pH-værdi: 7,5, Odourless, Liquid

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Kinase buffer I (5X)

Supplier: Thermo Fisher Scientific

Kinase buffer I (5X)

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Phosphate, 0,2 M buffer solution, pH 7,2

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH-værdi: 7,2, Liquid

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Carbonate-buffered saline (5X), pH 9,5

Supplier: Thermo Fisher Scientific

Cell culture buffer, Carbonate-buffered Salin, 5X

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Phosphate, 0,5M buffer solution, pH 8,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH-værdi: 8,5, Liquid

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Phosphate-buffered saline (PBS, 10X), with Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X), with Triton® X-100

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Triton® X-100 lysis buffer, pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

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IEF Cathode buffer (10X) with lysine and arginine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine and arginine

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Laemmli SDS sample buffer, non-reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)