Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Saltvand med fosfatbuffer (PBS), højt fosfatindhold

Supplier: VWR Chemicals

Strålebehandlet opl., pH 7,2, til skylleblots.

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VWR® Buffer Standard Solutions

Supplier: VWR Chemicals

Buffer Standard solution, Biotechnology Grade, pH-værdi: 10,0, ±0,03, Referencetemperatur: 25 °C, VWR®, Flaske

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VWR®, pH electrode maintenance kit

Supplier: VWR Chemicals

The measurement of the pH value is one of the most frequently and universally made measurements in science.

What is often not fully understood is the fact that a large number of problems related to pH measurement is the
correct selection, care or maintenance of the electrodes with particular emphasis on the reference electrode.

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions

Supplier: Avantor

Ready-to-use pH buffers are manufactured in large lots to stringent specifications and utilise quality control procedures to reduce lot-to-lot variability. You can eliminate the time and expense of preparation and standardisation of solutions, helping streamline high-volume analytical work in applications such as quality control.

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VWR®, Buffer, ammonia solution, pH 10 ready to use

Supplier: VWR Chemicals

Ideal for hardness of water determination.

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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

Supplier: VWR Chemicals

Sodium acetate buffer, VWR®, 3 M, pH-værdi: 5,2

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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J.T.Baker®, Sheath fluid, 3200/3400

Supplier: Avantor

Sheath fluid, 3200/4000, J.T.Baker®

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Bæredygtige muligheder

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VWR®, bufferløsninger, sekundære standarder

Supplier: VWR Chemicals

Buffer solutions, secondary standards, pH-værdi: 1,679, ±0,010, Referencetemperatur: 25 °C, VWR®, Plastflaske

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.