Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

Buffers, BIS-TRIS propane, 0,2 M solutions

Supplier: Thermo Fisher Scientific

2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxy methyl)propane-1,3-diol]

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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Lithium (Li) in 1% HNO₃

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Buffer, TRIS, 1,0 M Solution, pH 9,0

Supplier: Thermo Fisher Scientific

TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: LONZA

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Enzyme storage buffer in PBS and glycerol, autoclaved

Supplier: Thermo Fisher Scientific

Enzyme Storage Buffer in PBS and Glycerol

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ADA, 0,2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

N-(2-Acetimido)iminodiacetic acid monosodium salt (ADA) buffer solutions PH values 6,0 to 7,5.

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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Phosphate-buffered saline (PBS, 10X), no potassium

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), No potassium, 10X, Liquid

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IEF Sample buffer (4X) pH 3 to 7 with lysine

Supplier: Thermo Fisher Scientific

Loading Buffer, IEF Sample Buffer (4X) with Lysine

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, pH-værdi: 5,00, ±0.02, Ampul

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Phosphate buffer solution pH 7,00 (20 °C), Fluka™

Supplier: Honeywell Chemicals

Potassium dihydrogen phosphate / di-sodium hydrogen phosphate

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10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.