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Buffere

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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Buffer, TRIS, 1,0 M Solution, pH 9,0

Supplier: Thermo Fisher Scientific

TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid

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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: LONZA

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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Enzyme storage buffer in PBS and glycerol, autoclaved

Supplier: Thermo Fisher Scientific

Enzyme Storage Buffer in PBS and Glycerol

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, pH-værdi: 10,00, ±0.05, Ampul

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TE buffer, 1X, ready to use, pH 8,0, low EDTA, Molecular Biology Grade, Affynmetrix/USB

Supplier: Thermo Fisher Scientific

TE buffer with low EDTA. Use directly to re-suspend and/or dilute purified DNA or RNA.

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Phosphate-buffered saline (PBS, 10X), pH 7,4

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), 10X, pH-værdi: 7,4, Colourless, Liquid

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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Separating or resolving buffer (4X)

Supplier: Thermo Fisher Scientific

Separating Buffer, Separating or Resolving Buffer, 4X

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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Supplier: Thermo Fisher Scientific

Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

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IEF Cathode buffer (10X) with lysine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine

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Phosphate buffer solution pH 7,00 (20 °C), Fluka™

Supplier: Honeywell Chemicals

Potassium dihydrogen phosphate / di-sodium hydrogen phosphate

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G-Biosciences Tris-EDTA-NaCl Buffer

Supplier: G-Biosciences

 Tris-EDTA-NaCl buffer solution at pH 8,0.

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Buffer, PBS tablets, pH 7,4, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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