Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

Tris-Acetate-EDTA (TAE) buffer, 50X

Supplier: PanReac AppliChem

50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.

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G-Biosciences Tris Buffered Saline with Tween 20 (10X)

Supplier: G-Biosciences

A 10X concentrated Tris Buffered Saline supplemented with Tween 20.

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Phosphate-buffered saline (PBS, 10X), RNase free

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X, RNAse free

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Succinate, 0,2 M buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Succinate buffer solution, 0,2 M, pH-værdi: 6,5, Colourless, Odourless, Liquid

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Buffers, potassium phosphate, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Potassium phosphate, buffer solution, 0,5 M, pH-værdi: 7,0, Liquid

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Buffers, TRIS, 1,0 M solutions

Supplier: Thermo Fisher Scientific

Odourless, colourless TRIS buffer solution.

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Buffer solution for chlorine determination

Supplier: Hach

For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.

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HEPES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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HEPES-Buffered Saline, 5X Solutions

Supplier: Thermo Fisher Scientific

HEPES buffered saline, 5X, pH-værdi: 7,0, Odourless, Liquid

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Buffers, PIPES, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Liquid

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AccuGENE® PBS Buffers

Supplier: LONZA

AccuGENE™ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Semi Dry Blot Transfer buffer (10X)

Supplier: Thermo Fisher Scientific

Elektroforesebuffer, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Transfer or Electro Blotting buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Elektroforesebuffer, Transfer buffer, Transfer Electroblot Buffer 10X

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Buffers, sodium bicarbonate, 1 M solutions

Supplier: Thermo Fisher Scientific

Sodium bicarbonate buffer solution, 1,0 M, pH-værdi: 9,4, Liquid

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.