Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Phosphate-buffered saline (PBS, 10X), with Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X), with Triton® X-100

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Ammonia buffer solution pH10 (20 °C) for complexometry, Fluka™

Supplier: Honeywell Chemicals

Ammonia buffer

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Triton® X-100 lysis buffer, pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

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NP-40 lysis buffer with glycerol (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

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TRIS-buffered saline (TBS, 10X, high salt) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X, high salt)

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Phosphate, 0,2 M buffer solution, pH 8,0

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH-værdi: 8,0, Liquid

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M solution, pH 7,4, RNase free

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Citrat, pH-værdi: 3,00, ±0.02, Referencetemperatur: 20 °C, Fluka™, 6×1 L

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Tris-Acetate-EDTA (TAE) buffer, 50X

Supplier: PanReac AppliChem

50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.

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G-Biosciences Tris Buffered Saline with Tween 20 (10X)

Supplier: G-Biosciences

A 10X concentrated Tris Buffered Saline supplemented with Tween 20.

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Buffers, PIPES, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Liquid

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Semi Dry Blot Transfer buffer (10X)

Supplier: Thermo Fisher Scientific

Elektroforesebuffer, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X

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Buffers, sodium bicarbonate, 1 M solutions

Supplier: Thermo Fisher Scientific

Sodium bicarbonate buffer solution, 1,0 M, pH-værdi: 9,4, Liquid

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HEPES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.