Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

Buffer solutions, according to DIN 19266, Fluka™

Supplier: Honeywell Chemicals

Buffers with fungicide (except pH 10,012)

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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pH buffer solutions, Specpure®

Supplier: Thermo Fisher Scientific

pH Buffer solution, colour coded, Carbonat, pH-værdi: 10,00, ±0,01, Referencetemperatur: 25 °C, Specpure®, blå, Flaske

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Buffer Solutions

Supplier: Thermo Fisher Scientific

pH Buffer solution, pH-værdi: 7,00, ±0.01, Referencetemperatur: 25 °C

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Citrat, pH-værdi: 5,00, ±0.02, Referencetemperatur: 20 °C, Fluka™, 1×1 L

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Buffer solutions, colour coded, Fluka™

Supplier: Honeywell Chemicals

These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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Buffer, PBS tablets, pH 7,4, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.