Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Tris-Acetate-EDTA (TAE) buffer, 10X, molecular biology grade

Supplier: PanReac AppliChem

10X concentrated solution of 40 mM Tris, 20 mM glacial acetic acid and 1 mM EDTA.Na₂, pH 8,5 ±0,2.

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Tris-Borate-EDTA (TBE) buffer, 10X, Molecular biology grade

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: PanReac AppliChem

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Supplier: PanReac AppliChem

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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pH Buffer solutions

Supplier: Hach

For calibration of pH meters.

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pH buffer solutions, colour coded

Supplier: Hach

For calibration of pH meters

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pH buffers, DuraCal

Supplier: HAMILTON BONADUZ

For full information concerning health and safety data please contact Avantor or Hamilton directly.

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Genomic DNA extraction buffers

Supplier: OMEGA BIO-TEK

P2 buffer, Omega Bio-Tek

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Blocking buffers, protein-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce Protein-free blocking buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

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Buffer, HEPES solution pH 8 (1 mol/L)

Supplier: PanReac AppliChem

HEPES buffer solution pH 8 (1 mol/l)

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Buffer, HEPES solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

HEPES buffer solution pH 7,5 (1 mol/l)

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Tris-Glycine native gel running buffer

Supplier: G-Biosciences

Running Buffer, Tris-Glycine native gel running buffer

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Native sample loading buffer, 2X

Supplier: G-Biosciences

Loading Buffer, Native sample loading buffer (2X)

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Protein solubilisation buffer

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

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Tris Buffers, Sterile

Supplier: G-Biosciences

Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.