Buffere
Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.
Fixation Buffer (Flow Cytometry), Abcam
Supplier: Abcam
Fixation Buffer (ab314680) is a ready-to-use formaldehyde-based fixation buffer for immunofluorescence cell staining for microscopy or flow cytometry.
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Permeabilization Buffer (Flow Cytometry), Abcam
Supplier: Abcam
Permeabilization Buffer (ab314681) is a ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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Proteometer-CV reconstitution reagent
Supplier: NOVILYTIC
1 x vial of reconstitution reagent.
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Proteometer-CV fluorescent reagent
Supplier: NOVILYTIC
1 vial of Fluorescent reagent for the Proteometer-CV kit specifically formulated for use with Proteometer-CV kit.
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Proteometer-CV consumables kit
Supplier: NOVILYTIC
The Proteometer-CV is designed for the scientific development of mAbs, bi-specifics, or fusion proteins with a human Fc region, regardless of their stage in the development continuum. This kit offers an alternative to traditional charge variant methods that require Protein A.
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Proteometer-CV starter kit part 2/2 reagent (60 assays)
Supplier: NOVILYTIC
Fluorescent reagent for the Proteometer-CV Starter Kit.
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Proteometer-CV kit part 2/2 reagent (300 assays)
Supplier: NOVILYTIC
Fluorescent reagent for the Proteometer-CV kit.
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Blokerende buffere, SuperBlock®
Supplier: Thermo Fisher Scientific
Biotin- og serumproteinfri blokker Blokerer membraner hurtigt og effektivt, hvilket medfører høje signal/støjforhold.
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Pierce™ Fish Serum Blocking Buffer,Thermo Scientific
Supplier: Thermo Fisher Scientific
This Fish Serum blocking buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
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Mammalian protein extract reagents, M-PER™
Supplier: Thermo Fisher Scientific
The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.
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Nuclear and cytoplasmic extraction reagents, NE-PER
Supplier: Thermo Fisher Scientific
The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
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Y-PER™ Yeast Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.
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Binding and elution buffers for antibody purification, Pierce™
Supplier: Thermo Fisher Scientific
These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.
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Pierce™ BupH™ Wash Buffers, Dry Blend Pack
Supplier: Thermo Fisher Scientific
BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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Substrate buffers, Pierce™
Supplier: Thermo Fisher Scientific
These stable peroxide and diethanolamine substrate buffers produce optimised, reliable and convenient stock solutions for HRP and alkaline phosphatase assays detection respectively.
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Blocking buffer, Blocker™ BSA (10X) in PBS or TBS
Supplier: Thermo Fisher Scientific
Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
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