Buffere
Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.
TM Buffer, 1X Tris-Magnesium sulphate, pH 7,5
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.
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Diluent buffer, 10X concentrate, for dab membrane substrate
Supplier: Rockland Immunochemicals
Concentrate diluent buffer for DAB membrane substrate, 10X
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Competent cell buffer
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Rockland Immunochemicals
EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.
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GET buffer
Supplier: Rockland Immunochemicals
GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.
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PBS and TBS buffers
Supplier: Rockland Immunochemicals
Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)
Supplier: Rockland Immunochemicals
Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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2X SDS-PAGE sample buffer without DTT or b-ME
Supplier: Rockland Immunochemicals
Sample buffer for protein electrophoresis.
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Rockland Immunochemicals
This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
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SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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