Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Serva

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: Roth Carl

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

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EasySep™ Red Blood Cell Lysis Buffer

Supplier: Stemcell Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L)

Supplier: Serva

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Molecular Biology Grade

Supplier: Serva

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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ALDEFLUOR™ Assay Buffer

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Serva

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Fixation Buffer

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

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HEPES Buffer Solution (1 M)

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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RoboSep™-C PBS/EDTA Buffer

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

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Buffer, TRIS solution pH 8,8 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 8,8 (1 mol/l)

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

Supplier: Serva

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.