Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)

Supplier: Rockland Immunochemicals

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Supplier: Rockland Immunochemicals

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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TES buffer, 1X, ready to use, pH 7,8

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

TRK lysis buffer

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Buffer HF-Improved

Supplier: Thermo Fisher Scientific

Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.

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Dry buffer packs, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.

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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

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Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: G-Biosciences

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)

Supplier: G-Biosciences

Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.