Du søgte efter: Nucleic Acid Purification Kits and Reagents

Supplier: MP Biomedicals

MiniPrep Express™ Matrix is designed for rapid purification of plasmid DNA. It is an ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR.

Supplier: MP Biomedicals

SPINeasy® DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes.

Supplier: MP Biomedicals

The Fast DNA® SPIN Kit is used with the FastPrep®-24 or FastPrep® FP120 instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 30 minutes.

Supplier: MP Biomedicals

GeneClean® Turbo Kit is used for purification of DNA fragments of sizes 0,1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

Supplier: MP Biomedicals

The RNaid® Spin Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. The kit includes spin filters which prevent silica particle carry-over into cleaned RNA.

Supplier: MP Biomedicals

The Fast DNA® SPIN Kit for Faeces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

Supplier: MP Biomedicals

MPure™ Total RNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction of total RNA from whole blood, blood cells, animal tissue, plant tissue, yeast or cultured cells and viral RNA from human biological specimens such as serum, plasma, and other cell-free fluids.

Supplier: MP Biomedicals

MPure™ Blood DNA Extraction Kit is used with the MPure-12™ aNAP System for extraction of DNA from 100 to 400 µl mammalian whole blood, peripheral blood mononuclear cells or buffy coat. Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.

Supplier: MP Biomedicals

SPINeasy® DNA Kit for Tissue (with Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines mechanical, chemical and enzymatic disruption of tissue samples in 30 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.

Supplier: MP Biomedicals

MPure-32™ aNAP System is a fully automated magnetic bead-based nucleic acid extraction system. It can process up to 32 samples simultaneously within a short period of time (around 40 to 60 minutes). Besides its capability to extract nucleic acid with high purity and yield, MPure-32™ aNAP System is easy to use; this eliminates the risk of human error and cross-contamination.

Supplier: MP Biomedicals

MPure™ Plant DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction of genomic DNA from plant (leaf, seeds and spores) and fungal tissues. Up to 100 mg of tissue can be used for purification.

Supplier: MP Biomedicals

FastRNA® Pro Green Kit is used to isolate total RNA from any type of plant and animal tissue or cultured cells. Extracted RNA is suitable for RT-PCR analysis and other downstream applications.

Supplier: Biotium

The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.

Supplier: G-Biosciences

Yeast Geno-DNA Template™ isolates high quality genomic DNA from yeast. The kit is based on a two-step lysis of yeast cells, using LongLife™ Zymolyase® and LongLife™ Proteinase K, followed by the removal of proteins and other cellular impurities by precipitation and centrifugation.

Supplier: G-Biosciences

Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, in vitro translation, RNase protection assays and poly (A+) selection.

Supplier: G-Biosciences

OmniPrep™ for Tissue Kit is to specifically isolate high quality genomic DNA from tissue samples, including fresh, frozen, fixed or paraffin-embedded tissue. The kit isolates DNA from bodily fluids, including plasma, serum, amniotic fluid, semen and CSF. Also isolates high quality DNA from cultured cells, non-mammalian nucleated blood and gram-negative bacteria.

Supplier: Thermo Fisher Scientific

GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Supplier: Thermo Fisher Scientific

Applications: Complete and ready to use reagent for the isolation of genomic DNA from solid and liquid samples of animal and plant origin

Supplier: G-Biosciences

GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Supplier: G-Biosciences

G-Biosciences' GeneCAPSULE™ is an electro-elution tool for rapid, nearly 90% recovery of PCR products, DNA fragments and proteins from agarose and polyacrylamide gels

Supplier: Thermo Fisher Scientific

GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Supplier: G-Biosciences

OmniTemplate™ is specifically designed for the rapid isolation of a DNA template for PCR analysis from mammalian tissue samples, blood and cell cultures. This single tube method produces a high concentration, ready-to-use supply of DNA template for microanalysis, genotyping and a multitude of other applications.

Supplier: Thermo Fisher Scientific

GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Supplier: Merck Millipore (Novagen)

Pellet Paint® NF Co-Precipitant is a non-fluorescent dye-labelled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye® Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualised carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labelled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Re-suspension of pelleted sequencing reaction products in deionised formamide can be confirmed by checking for dissolution of the carrier pellet.

Supplier: G-Biosciences

OmniPrep™ for Yeast Kit isolates high quality genomic DNA from yeast. When used according to the recommended protocols, this kit purifies DNA from 300 ml yeast culture.

Supplier: G-Biosciences

OmniPrep™ for Plant Kit isolates high quality genomic DNA from plant samples, including fresh and frozen plant tissues. If used according to the protocols this kit purifies DNA from 20gm plant tissue.

Supplier: G-Biosciences

OmniPrep™ for Blood Kit isolates high quality genomic DNA from blood samples, including whole blood, buffy coats, packed cells and bone marrow.

Supplier: Merck Millipore (Novagen)

Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Supplier: Merck Millipore (Novagen)

The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.