RT-PCR og cDNA-reagenser

Nukleinsyrereagenser danner grundlaget for genetisk analyse og molekylærbiologi og spiller en afgørende rolle fra kloning til genekspressionsstudier. Udforsk vores kollektion med højpræcisionsenzymer til end-point PCR, avancerede reagenser til isothermal amplifikation og omfattende kits til oprensning af nukleinsyrer. For præcision i kvantitative assays, se vores udvalg af qPCR- og RT-qPCR-kits, designet til enestående nøjagtighed. Vores portefølje inkluderer også reagenser til næste generations sekventering, som tilbyder banebrydende løsninger til genomsekventering og fremskridt inden for personlig medicin.

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One step RT-PCR master mix kit

Supplier: Merck Millipore (Novagen)

The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

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First-Strand cDNA Synthesis Kit

Supplier: Cytiva

Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

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RT-PCR beads, illustra™ Ready-To-Go™

Supplier: Cytiva

Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.

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PCR beads, Ready-To-Go™ You-Prime First-Strand Beads

Supplier: Cytiva

Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.