Primere og prober

Magnetiske omrøringsstænger giver effektiv omrøring af prøver via magnetisk manipulation. Mange modeller har en ottekantet form med en central integreret drejering for optimal turbulens under omrøring takket være større overfladeareal. De er ofte belagt med teflon for at give kemisk inaktivitet; lejlighedsvis vil glas være belægningen til brug med de fleste flydende alkalimetaller og alkalimetalløsninger i ammoniak. Begge disse belægninger er inerte og vil ikke forurene eller reagere med reaktionsopløsningen.

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Universal reverse primer

Supplier: AGILENT

The universal reverse primer serves as the downstream primer in the qPCR reaction by annealing to a universal sequence tag that is added to the cDNA during the reverse transcription protocol.

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Random hexamer primer

Supplier: Thermo Fisher Scientific

Random hexamer primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Random hexamer primer has been functionally tested in first strand cDNA synthesis.

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Oligo(dT)₁₈ primer

Supplier: Thermo Fisher Scientific

Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.

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PCR primers, Mouse Ig-primer set

Supplier: Merck Millipore (Novagen)

The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.