Transfektionsreagenser
Avantor tilbyder transfektionsreagenser til adskillige anvendelser, herunder bioproduktion, in vivo-arbejde, virusproduktion, DNA- og siRNA-transfektion, CRISPR/Cas 9 og mere. Når man overvejer et passende transfektionsreagens, er det vigtigt at identificere celletypen og dyrkningsbetingelserne. Specialiserede transfektionsreagenser vil være rettet mod sjældne cellekulturer, neuroner og primære celler, der er vanskeligere at manipulere for at opnå porøse egenskaber. Avantor tilbyder også produkter til elektroporation og de forbrugsvarer, der er nødvendige for at understøtte transfektionsarbejdsgangen.
Transfection reagent, TurboFect in-vitro
Supplier: Thermo Fisher Scientific
TurboFect transfection reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.
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mRNA transfection reagents, RmesFect™
Supplier: OZ BIOSCIENCES
RmesFect™ transfection reagent is based on the TEE-technology and is specifically designed for mRNA transfection with high efficiency and low toxicity. It is suitable for in vitro mRNA (dsRNA) transfection in a large variety of cells.
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Protein transfection reagent, Novagen®, ProteoJuice™
Supplier: Merck Millipore (Novagen)
ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.
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Transfection reagent, Insect GeneJuice®
Supplier: Merck Millipore (Novagen)
Insect GeneJuice® Transfection Reagent is formulated to maximise transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for co-transfection of transfer plasmids with linearised virus DNA for the production of recombinant baculoviruses.
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Bipolar transfection system, HBIO AgilePulse PLUS
Supplier: BTX
Easily perform advanced transfection protocols with multiple groups of pulses-each with separate parameters.
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Transfection kit, BacMagic™
Supplier: Merck Millipore (Novagen)
The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
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Transfection reagent, 293-Free™
Supplier: Merck Millipore (Novagen)
293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.
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DNA kit, BacMagic™-2
Supplier: Merck Millipore (Novagen)
The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
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siRNA transfection reagent, RiboJuice™
Supplier: Merck Millipore (Novagen)
RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.
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Transfection kit, BacMagic™-2
Supplier: Merck Millipore (Novagen)
The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
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dNTP Mix (10 mM)
Supplier: Stemcell Technologies
A solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM.
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Transfektionsreagenser, DharmaFECT® Duo
Supplier: Horizon Discovery
Et lipid-baseret reagens, som er specielt sammensat til co-transfektion af plasmid og siRNA.
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ArciTect™ crRNA
Supplier: Stemcell Technologies
Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing.
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ArciTect™ tracrRNA Kit
Supplier: Stemcell Technologies
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing.
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STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
Supplier: Stemcell Technologies
For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing.
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ArciTect™ T7 Endonuclease I Kit
Supplier: Stemcell Technologies
For estimation of CRISPR-Cas9 genome editing efficiency.



