Running-buffere

Elektroforesebufferne opretholder ensartede pH-niveauer under separation af DNA, RNA og andre proteiner. Lavladningsopløsningerne leverer ioner, der overfører den påførte strøm, og gør det muligt for forskellige molekylstørrelser at bevæge sig med forskellige hastigheder under analyser. De præparative stoffer, der giver yderligere beskyttelse til proteiner, forhindrer nedbrydning for at garantere prøvelevedygtighed og nøjagtig testning. Den bedste elektroforesebuffer, der fås i forskellige molekylvægte, kan vælges baseret på de specifikke makromolekyler, der håndteres.

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

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G-Biosciences TAE Running Buffer (50X)

Supplier: G-Biosciences

The primary use of TAE Running Buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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SDS-Page running gel buffers, 10X concentrate

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

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Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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G-Biosciences MOPS Running Buffers (10X)

Supplier: G-Biosciences

A zwitterionic buffer providing consistent buffering capacity in the neutral range, ideal for RNA electrophoresis.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).