Running-buffere

Elektroforesebufferne opretholder ensartede pH-niveauer under separation af DNA, RNA og andre proteiner. Lavladningsopløsningerne leverer ioner, der overfører den påførte strøm, og gør det muligt for forskellige molekylstørrelser at bevæge sig med forskellige hastigheder under analyser. De præparative stoffer, der giver yderligere beskyttelse til proteiner, forhindrer nedbrydning for at garantere prøvelevedygtighed og nøjagtig testning. Den bedste elektroforesebuffer, der fås i forskellige molekylvægte, kan vælges baseret på de specifikke makromolekyler, der håndteres.

Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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TBE running buffer (10X)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE running buffer (10X)

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Tris base buffer, powder, Corning®

Supplier: Corning

Tris is commonly used in buffer solutions in biochemical and molecular biological research.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5

Supplier: PanReac AppliChem

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5

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Buffer, PBS (1X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (1X) according to Dulbecco.

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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8,3

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-Tricine-SDS running buffer (10X), cathode buffer

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IEF Cathode buffer (10X) with lysine and arginine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine and arginine

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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DIGE buffer kit

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

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IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.