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Ekstraktions buffere

Ekstraktions buffere

Ekstraktionsbuffere leveres klar til brug og er effektive til isolering af total RNA og andre proteiner, hvilket forbedrer proteinudbyttet og prøvekompleksiteten fra celler og væv. Optimerede ekstraktionsreagenser forbedrer proteinudtræk i native eller denaturerende buffere, der er kompatible med efterfølgende analyseteknikker. En passende buffer opløsning tilsat til en proteinblanding under ekstraktionen kan hjælpe med at stabilisere proteinmolekylerne, mens de udsættes for forskellige kræfter, der skal isolere dem til videre undersøgelse.

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Extraction Enhancer Buffer 50X, Abcam

Supplier: Abcam

Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays.

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RIPA Buffer, Abcam

Supplier: Abcam

A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.

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HeLa Nuclear Extract, Abcam

Supplier: Abcam

Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

    
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RBC Lysis Buffer (10X)

RBC Lysis Buffer (10X)

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam

Supplier: Abcam

Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.

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Cell Lysis Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

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10X RIPA Buffer, Abcam

Supplier: Abcam

10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins

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Cell lysis analysis kit, SideStep II

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

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G-Biosciences Red Blood Cell Lysis Buffer

Supplier: G-Biosciences

A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

    
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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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Gram Negative lysis buffer

Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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1X Red Blood Cell Lysis Buffer, Abcam

Supplier: Abcam

Red Blood Cell (RBC) Lysis Buffer ab286858 provides a quick and efficient method of lysing red blood cells. It is provided in a 1x concentrated format, and is also available as ab204733 in a 10x format.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.

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