Assay buffere
Assay-buffere er blandinger af buffere, salte, detergenter, protein, konserveringsmidler og farvestoffer. Disse buffere bruges i ELISA-assays til fortynding af standarder, prøver og reagenser samt til blokering af uspecifikke interaktioner. Buffere kan bruges individuelt eller i kombination. Blokeringsbuffere er specifikt designet til at bevare proteiners konformationelle rumlige struktur i deres naturlige foldede tilstand og dermed sikre proteinets biologiske aktivitet.
Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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ALDEFLUOR™ Assay Buffer
Supplier: Stemcell Technologies
Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.
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