Vaskebuffere

Elektroforesepuffer opretholder stabile pH-niveauer under separation af DNA, RNA og andre proteiner. De leverer ioner, som leder den påførte strøm, og deres lave ladning gør det muligt for molekyler af forskellige størrelser at bevæge sig i forskellige hastigheder under analysen. For at beskytte proteiner yderligere forhindrer de forberedende stoffer nedbrydning, hvilket sikrer prøvens levedygtighed og præcise resultater. Fås i forskellige molekylvægte, og den bedste buffer kan vælges afhængigt af de specifikke makromolekyler.

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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TRIS-buffered saline (TBS, 10X, high salt) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X, high salt)

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Imidazole-buffered saline (5X)

Supplier: Thermo Fisher Scientific

Washing buffer, Imidazole-buffered saline (5X)

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Phosphate-buffered saline (PBS, 10X), with Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X), with Triton® X-100

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Tween 20 washing buffer, 2% in PBS (20X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 2% in PBS (20X)

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals

Supplier: MP Biomedicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Wash Solution Concentrate, 20X

Supplier: SERACARE LIFE SCIENCES

Convenient 20X concentrate containing imidazole-buffered saline and Tween® 20.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: Roth Carl

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR®, Nitril handsker, NITRILE

#: 112-2372

: VWR Collection

VWR®, Transfer Pipettes, High Performance

#: 612-1681

: VWR Collection

Acetonitril, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

#: 83639.320

: VWR Chemicals

Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

#: 20864.320

: VWR Chemicals

VWR®, Serologiske pipetter, Standard type

#: 612-3700

: VWR Collection

Acetone ≥99%, TECHNICAL

#: 20063.365

: VWR Chemicals

VWR® NITRILE LIGHT, Nitrile Gloves

#: 112-2754

: VWR Collection

VWR®, Ansigtsservietter

#: 115-0600

: VWR Collection

Sealing film for manual application, Parafilm® M

#: 291-0057

: HEATHROW

Handsker, nitril, Kimtech™ Purple Nitrile™

#: 112-4847

: Ansell

VWR®, Laboratorieflasker, rund, med skruelåg

#: 215-1592

: VWR Collection

Acetonitril ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

#: 83640.320

: VWR Chemicals

Natriumhydroxid 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

#: 31770.294

: VWR Chemicals

pH-teststrimler, MQuant®, Supelco®

#: 1.09535.0001

: Merck

Acetonitril ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

#: 1.00030.2500

: Merck

Sprøjter, 2-delte, HENKE-JECT®

#: 613-2001

: HENKE SASS WOLF

VWR® AVS TITRINORM, bufferopløsninger, 20 °C

#: 32096.291

: VWR Chemicals

Vand, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

#: 83645.320

: VWR Chemicals

2-Propanol ≥99.8%, HiPerSolv CHROMANORM® for HPLC

#: 20880.320

: VWR Chemicals